Anti-DYRK1A 抗体 (ab69811)

製品の概要

  • 製品名Anti-DYRK1A antibody
    DYRK1A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DYRK1A
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human DYRK1A aa 733-752 conjugated to keyhole limpet haemocyanin.
    Sequence:

    TEGHLTMRQGADREESPMTG


    Database link: Q13627

  • ポジティブ・コントロール
    • Rat brain postnatal and embryonic extracts.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 86 kDa.

ターゲット情報

  • 機能May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates.
  • 組織特異性Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
  • 関連疾患Defects in DYRK1A are the cause of mental retardation autosomal dominant type 7 (MRD7) [MIM:614104]. A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
    Contains 1 protein kinase domain.
  • 発生段階Expressed in the developing central nervous system. Overexpressed 1.5-fold in fetal Down syndrome brain.
  • ドメインThe polyhistidine repeats act as targeting signals to nuclear speckles (PubMed:19266028).
  • 翻訳後修飾Autophosphorylated on tyrosine residues.
  • 細胞内局在Nucleus speckle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dual specificity tyrosine phosphorylation regulated kinase antibody
    • Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A antibody
    • Dual specificity tyrosine phosphorylation regulated kinase 1 antibody
    • Dual specificity tyrosine phosphorylation regulated kinase 1A antibody
    • Dual specificity tyrosine-phosphorylation-regulated kinase 1A antibody
    • Dual specificity YAK 1 related kinase antibody
    • Dual specificity YAK1 related kinase antibody
    • Dual specificity YAK1-related kinase antibody
    • DYR1A_HUMAN antibody
    • DYRK 1 antibody
    • DYRK 1A antibody
    • DYRK antibody
    • DYRK1 antibody
    • Dyrk1a antibody
    • DYRKA antibody
    • hMNB antibody
    • HP 86 antibody
    • HP86 antibody
    • Minibrain (Drosophila) homolog antibody
    • Minibrain homolog antibody
    • Minibrain, Drosophila, homolog of antibody
    • MNB antibody
    • MNB protein kinase antibody
    • Mnb protein kinase homolog hp86 antibody
    • MNB protein kinase, Serine/threonine-specific antibody
    • MNB/DYRK protein kinase antibody
    • MNBH antibody
    • MRD7 antibody
    • OTTHUMP00000109090 antibody
    • OTTHUMP00000109091 antibody
    • OTTHUMP00000109094 antibody
    • OTTHUMP00000174799 antibody
    • Protein kinase minibrain homolog antibody
    • Serine/threonine kinase MNB antibody
    see all

Anti-DYRK1A antibody 画像

  • Anti-DYRK1A antibody (ab69811) at 1/1000 dilution + 30µg of HeLa whole cell lysate

    Secondary
    Donkey anti-Rabbit conjugated to HRP at 1/5000 dilution

    Predicted band size : 86 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-DYRK1A antibody (ab69811) 使用論文

ab69811 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Chicken Cell lysate - whole cell (DF-1)
Loading amount 30 µg
Specification DF-1
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Feb 18 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Feb 11 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jan 27 2009

Thank you for your enquiry. ab16140:The antigen made to generate the antibody is specific to Dyrk1A. That said, we have never actually tested against any other Dyrk protein, but the Dyrk1A sequence differs so greatly from Dyrk1B that it would be hig...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"