Anti-Dynorphin A 抗体 (ab82509)


  • 製品名Anti-Dynorphin A antibody
    Dynorphin A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dynorphin A
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Guinea pig, Cow, Pig, Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Dynorphin A.

    (Peptide available as ab91634.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human brain membrane tissue lysate.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab82509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).


  • 関連性Dynorphins are a class of peptides produced by many different populations of neurons, which has some opiate like activity. It is thus classed as an endogenous opioid peptide. The dynorphins, which include dynorphin A, dynorphin B, alpha and beta neoendorphin, and big dynorphin, are all the products of a single gene, 'preprodynorphin'. Dynorphin is produced in many different parts of the brain, including the hypothalamus, the hippocampus and the spinal cord, and has many different physiological actions, depending upon its site of production. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. As such, it may help some individuals against addiction. Blocking dynorphin may help alleviate depression.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • Beta neoendorphin dynorphin antibody
    • Dyn A17 antibody
    • Dynorphin A antibody
    • PDYN antibody
    • Preprodynorphin antibody
    • Proenkephalin B antibody
    see all

Anti-Dynorphin A antibody 画像

  • Anti-Dynorphin A antibody (ab82509) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

    Exposure time : 15 minutes
  • ICC/IF image of ab82509 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 5µg/ml.
  • IHC image of Dynorphin A staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82509, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • Anti-Dynorphin A antibody (ab82509) at 1/1000 dilution + Mouse synaptosome from hippocampal stratum lucidum tissue lysate at 10 µg

    HRP-conjugated anti-rabbit IgG polyclonal at 1/5000 dilution
    developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 56 kDa (possible non-specific binding),65 kDa (possible non-specific binding).

    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Dynorphin A antibody (ab82509) 使用論文

ab82509 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Non-reduced Denaturing
Sample Mouse Tissue lysate - other (synaptosome from hippocampal stratum lucidum)
Specification synaptosome from hippocampal stratum lucidum
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Apr 23 2014

Thank you for your call yesterday and for your question about these antibodies. I apologize for the delay getting back to you.

We have a general IHC protocol for FFPE sections that includes a section on antigen retrieval-


Read More