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RabMAb

Anti-DYNLL1 抗体 [EP1660Y] (ab51603)

製品の概要

  • 製品名
    Anti-DYNLL1 antibody [EP1660Y]
    DYNLL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1660Y] to DYNLL1
  • 特異性
    ab51603 recognizes DLC8. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • アプリケーション
    適用あり: Flow Cyt, WB, IHC-P, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to Human DYNLL1 aa 1-100 (N terminal).

  • エピトープ
    The epitope for this antibody is on the N-terminus, AA2-14.
  • ポジティブ・コントロール
    • WB: HeLa cell lysate. IHC-P: Human liver tissue. Flow Cyt: HeLa cells.
  • 特記事項

    Alternative versions available:

    Anti-DYNLL1 antibody (Alexa Fluor® 488) [EP1660Y] (ab203988)

    Anti-DYNLL1 antibody (HRP) [EP1660Y] (ab204008)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/2300.
WB 1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P 1/500.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

IP 1/30.

For unpurified use at 1/100.

ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能
    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.
    Binds and inhibits the catalytic activity of neuronal nitric oxide synthase.
    Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.
    Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the dynein light chain family.
  • 翻訳後修飾
    Phosphorylation at Ser-88 appears to control the dimer-monomer transition. According to PubMed:15193260, it is phosphorylated at Ser-88 by PAK1, however, according to PubMed:18650427, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro.
  • 細胞内局在
    Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Upon induction of apoptosis translocates together with BCL2L11 to mitochondria.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • 8 kDa dynein light chain antibody
    • 8kDLC antibody
    • Cytoplasmic dynein light polypeptide antibody
    • DLC1 antibody
    • DLC8 antibody
    • DNCL1 antibody
    • DNCLC1 antibody
    • DYL1_HUMAN antibody
    • Dynein , cytoplasmic, light chain 1 antibody
    • Dynein light chain 1 cytoplasmic antibody
    • Dynein light chain 1, cytoplasmic antibody
    • Dynein light chain LC8 type 1 antibody
    • Dynein light chain LC8-type 1 antibody
    • Dynein, cytoplasmic, light polypeptide 1 antibody
    • Dynein, light chain, LC8-type 1 antibody
    • DYNLL1 antibody
    • HDLC1 antibody
    • LC8 antibody
    • LC8a antibody
    • MGC126137 antibody
    • MGC126138 antibody
    • MGC72986 antibody
    • PIN antibody
    • Protein inhibitor of neuronal nitric oxide synthase antibody
    • Protein inhibitor of neuronal NOS antibody
    see all

画像

  • All lanes : Anti-DYNLL1 antibody [EP1660Y] (ab51603) at 1/5000 dilution (unpurified)

    Lane 1 : Primary mouse Mb1-Cre control Eµ-Myc B cell lymphoma (lysate of whole lymphnode)
    Lane 2 : Primary mouse Mb1-Cre Dynll1-conditional knockout Eµ-Myc B cell lymphoma (lysate of whole lymphnode)

    Secondary
    HRP conjugated polyclonal goat IgG at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 10 kDa
    Observed band size : 10 kDa


    Exposure time : 10 minutes

    This image is courtesy of an abreview submitted by Dr. Jörg Heierhorst

    Lymphnodes were dissociated in PBS 2% FBS. Cell suspensions filtered through 70 µm and 40 µm cell strainers, and 300 x g pellets were lysed in modified RIPA buffer (150 mM NaCl, 20 mM Tris pH7.4, 1 mM EDTA, 1 mM EGTA, 10 mM NaF, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 1 mM PMSF, 1 x protein inhibitor cocktail (Sigma)).

    See Abreview

  • Immunohistochemical staining of paraffin embedded human liver using unpurified ab51603 (1/100).

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling DYNLL1 (red) with purified ab51603 at a 1/2300 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Anti-DYNLL1 antibody [EP1660Y] (ab51603) at 1/10000 dilution (unpurified) + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 10 kDa
    Observed band size : 10 kDa
  • Unpurified ab51603 staining DLC8 in mouse kidney cells cells by ICC/IF (immunocytochemistry/immunofluorescence. Cells were fixed with methanol, permeabilized with 0.1% Triton and blocked with 1% milk for 1 hour at room temperature. The sample was incubated with primary antibody (1/400; 1% milk in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG (1/1000) was used as secondary antibody.

    See Abreview

  • Unpurified ab51603 used in IP.SKAP and Astrin form a complex. (A, left) Silver-stained gels showing a one-step IP of GFPLAP-Astrin, GFPLAP-SKAP, or GFPLAP-LC8. (A, right) Data from the mass spectrometric analysis of the purifications indicating the percent sequence coverage from each IP. (B) Silver-stained gel showing the purification of FLAG-SKAP from chicken DT40 cells relative to controls. The indicated proteins were identified by excising them from a gel and analyzing them by mass spectrometry.

参考文献

This product has been referenced in:
  • Wong DM  et al. The Transcription Factor ASCIZ and Its Target DYNLL1 Are Essential for the Development and Expansion of MYC-Driven B Cell Lymphoma. Cell Rep 14:1488-99 (2016). Read more (PubMed: 26832406) »
  • Jayappa KD  et al. Human immunodeficiency virus type 1 employs the cellular dynein light chain 1 protein for reverse transcription through interaction with its integrase protein. J Virol 89:3497-511 (2015). WB, IP, Flow Cyt ; Human . Read more (PubMed: 25568209) »

See all 11 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 24 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (B cell lymphoma lymphnodes)
Gel Running Conditions
Reduced Denaturing (15% midigel)
Loading amount
12 µg
Specification
B cell lymphoma lymphnodes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Jörg Heierhorst

Verified customer

投稿 Apr 13 2016

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (15% GEL)
Sample
Mouse Cell lysate - whole cell (mouse Embryonic Fibroblasts)
Specification
mouse Embryonic Fibroblasts
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 May 30 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15% GEL)
Sample
Human Cell lysate - other (H23)
Specification
H23
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Dec 13 2013

Code de remise : xxxxxx
Date d’expiration : 30/11/12

Je suis ravie que vous souhaitiez participer a notre offre et tester ab51603 chez la drosophile. Le code de réduction correspondant est xxxxxxxxx. Ce code de réduc...

Read More

Merci de votre intérêt pour ab51603. A ma connaissance, cet anticorps n’a pas encore été testé chez la Drosophile et nous n’offrons pas d’échantillon gratuit à des fins de tests pr&eacut...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Recombinant protein (Recombinant protein cloned into EcoR1)
Loading amount
0.5 µg
Specification
Recombinant protein cloned into EcoR1
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jul 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
20 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (4-12 % gradient gel, Novex)
Username

Abcam user community

Verified customer

投稿 Mar 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Kidney)
Specification
Kidney
Fixative
Methanol
Permeabilization
Yes - 0.1 % Triton
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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