Anti-DYNLL1 抗体 (ab92936)

製品の概要

  • 製品名Anti-DYNLL1 antibody
    DYNLL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DYNLL1
  • アプリケーション適用あり: WB, IHC-FoFr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Cow, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human DLC8.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse brain and hippocampus tissue lysates, Human brain tissue lysate and in the following whole cell lysates: MDA-MB-231; MCF7; PC12; HeLa.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.
    Binds and inhibits the catalytic activity of neuronal nitric oxide synthase.
    Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.
    Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the dynein light chain family.
  • 翻訳後修飾Phosphorylation at Ser-88 appears to control the dimer-monomer transition. According to PubMed:15193260, it is phosphorylated at Ser-88 by PAK1, however, according to PubMed:18650427, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro.
  • 細胞内局在Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Upon induction of apoptosis translocates together with BCL2L11 to mitochondria.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 8 kDa dynein light chain antibody
    • 8kDLC antibody
    • Cytoplasmic dynein light polypeptide antibody
    • DLC1 antibody
    • DLC8 antibody
    • DNCL1 antibody
    • DNCLC1 antibody
    • DYL1_HUMAN antibody
    • Dynein , cytoplasmic, light chain 1 antibody
    • Dynein light chain 1 cytoplasmic antibody
    • Dynein light chain 1, cytoplasmic antibody
    • Dynein light chain LC8 type 1 antibody
    • Dynein light chain LC8-type 1 antibody
    • Dynein, cytoplasmic, light polypeptide 1 antibody
    • Dynein, light chain, LC8-type 1 antibody
    • DYNLL1 antibody
    • HDLC1 antibody
    • LC8 antibody
    • LC8a antibody
    • MGC126137 antibody
    • MGC126138 antibody
    • MGC72986 antibody
    • PIN antibody
    • Protein inhibitor of neuronal nitric oxide synthase antibody
    • Protein inhibitor of neuronal NOS antibody
    see all

Anti-DYNLL1 antibody 画像

  • All lanes : Anti-DYNLL1 antibody (ab92936) at 1 µg/ml

    Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Mouse Hippocampus Tissue Lysate
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 6 : Human brain tissue lysate - total protein (ab29466)
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 10 kDa
    Observed band size : 10 kDa


    Exposure time : 90 seconds

    Secondary antibody goat anti-rabbit HRP (ab97080)

  • ICC/IF image of ab92936 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92936, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 1µg/ml.

Anti-DYNLL1 antibody (ab92936) 使用論文

ab92936 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: 21°C
Antigen retrieval step None
Sample Mouse Tissue sections (Cervical spinal cord, ventral horn)
Specification Cervical spinal cord, ventral horn
Permeabilization Yes - 0.1% Triton X
Fixative Paraformaldehyde
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Laura

Verified customer

投稿 Sep 24 2013

Though the images looks the same however if carefully observed, these are different. These antibodies are different because they are raised using different sequence of immunogen. I can re-assure you that both of antibodies would detect the endogenous f...

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