Anti-Dynein heavy chain 抗体 [440.4] (ab6305)

製品の概要

  • 製品名Anti-Dynein heavy chain antibody [440.4]
    Dynein heavy chain 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [440.4] to Dynein heavy chain
  • 特異性The antibody identifies the cytoplasmic dynein heavy chain (440 kDa) in immunoblotting with dynein enriched preparations and in crude cell extracts of chicken brain and fibroblasts.
  • アプリケーション適用あり: IP, WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Rat, Chicken, Human
    非交差種: Hamster
  • 免疫原

    Cytoplasmic full length native protein (purified from chick brain) (Chicken).

  • 特記事項


    Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These protein "motors" contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile funtions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD) (Vallee RB & Shpetner.).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 15mM Sodium Azide
  • Concentration information loading...
  • 精製度Ascites
  • 一次抗体 備考Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These protein "motors" contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile funtions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD) (Vallee RB & Shpetner.).
  • ポリ/モノモノクローナル
  • クローン名440.4
  • ミエローマunknown
  • アイソタイプIgG2a
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.
WB 1/100.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution. PubMed: 20890278

ターゲット情報

  • 機能Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
  • 配列類似性Belongs to the dynein heavy chain family.
  • ドメインDynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
  • 細胞内局在Cytoplasm, cytoskeleton, cilium axoneme.
  • Information by UniProt
  • 参照データベース
  • 別名
    • axonemal antibody
    • Axonemal beta dynein heavy chain 9 antibody
    • Ciliary dynein heavy chain 9 antibody
    • Ciliary dynein heavy chain antibody
    • DNAH 9 antibody
    • DNAH17L antibody
    • DNAH9 antibody
    • DNAH9 variant protein antibody
    • Dnahc 9 antibody
    • Dnahc9 antibody
    • DNAL 1 antibody
    • DNAL1 antibody
    • DNEL 1 antibody
    • DNEL1 antibody
    • DYH 9 antibody
    • DYH9 antibody
    • DYH9_HUMAN antibody
    • Dynein axonemal heavy chain 9 antibody
    • Dynein axonemal heavy polypeptide 17 like antibody
    • Dynein axonemal heavy polypeptide 9 antibody
    • Dynein heavy chain 9 antibody
    • dynein heavy chain 9, axonemal antibody
    • HL 20 antibody
    • HL20 antibody
    • KIAA0357 antibody
    see all

Anti-Dynein heavy chain antibody [440.4] 画像

  • All lanes : Anti-Dynein heavy chain antibody [440.4] (ab6305) at 1/1500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Rat whole brain tissue lysate at 3000 µg

    Secondary
    IRDye®680 donkey anti mouse polyclonal at 1/10000 dilution

    Performed under non-reducing conditions.

    Exposure time : 1 hour

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab6305 staining Dynein heavy chain in rat hippocampal neurons by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol, permeabilised in 0.1% Triton and then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Alexa Fluor® 488 used at a 1/1000 dilution.
    Left image stained with tubulin.
    Right image stained with ab6305.

Anti-Dynein heavy chain antibody [440.4] (ab6305) 使用論文

This product has been referenced in:

See all 5 Publications for this product

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I am very pleased to hear you would like to accept our offer and testthese antibodiesin mouse. This code will give you 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview formouse and include th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat Hippocampal Neuron)
Specification Rat Hippocampal Neuron
Fixative Methanol
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Apr 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Whole Brain Lysate)
Loading amount 3000 µg
Specification Whole Brain Lysate
Gel Running Conditions Non-reduced Denaturing (Tris-glycine 4-20% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Dec 07 2009

Application Immunoprecipitation
Sample African Green Monkey Cell lysate - whole cell (cos7 cells)
Total protein in input 6000 µg
Specification cos7 cells
Immuno-precipitation step Protein A
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投稿 Nov 16 2009

Thank you for your e-mail and for taking your time to fill in the Questionnaire. I am very sorry that you are experiencing problem with this antibody. My colleague Miss Charlotte Milnes has informed me (she was away for a couple of days) that you h...

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This antibody is raised in mouse so is not expected to cross-react with the mouse protein, although this has not been specifically determined.

The antibody identifies the cytoplasmic dynein heavy chain (440 kDa) in immunoblotting. Its specificity with DHC isoforms has not been tested.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"