Anti-DYNC2H1 抗体 (ab122525)
Key features and details
- Rabbit polyclonal to DYNC2H1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-DYNC2H1 antibody
DYNC2H1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DYNC2H1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
amino acids 3954-4051 (DVFNQRNKKS IFPYSVSLPQ SCSILDYRAV IEKIPEDDKP SFFGLPANIA RSSQRMISSQ VISQLRILGR SITAGSKFDR EIWSNELSPV LNLWKKLN) of Human DYNC2H1.
-
ポジティブ・コントロール
- Human small intestine.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glucose -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122525の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
May function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis. May play a role in transport between endoplasmic reticulum and Golgi or organization of the Golgi in cells. -
関連疾患
Defects in DYNC2H1 are the cause of asphyxiating thoracic dystrophy type 3 (ATD3) [MIM:613091]. ATD3 is an autosomal recessive osteochondrodysplasia which often leads to death in infancy because of a severely constricted thoracic cage and respiratory insufficiency.
Defects in DYNC2H1 are the cause of short rib-polydactyly syndrome type 3 (SRPS3) [MIM:263510]; also called Verma-Naumoff syndrome. A lethal skeletal dysplasia characterized by markedly short ribs, short limbs, polydactyly, narrow thorax, and multiple anomalies of major organs, including heart, intestines, genitalia, kidney, liver, and pancreas. -
配列類似性
Belongs to the dynein heavy chain family. -
細胞内局在
Cytoplasm > cytoskeleton > cilium axoneme. Cell membrane. Cytoplasm. Localizes to the apical cytoplasm (By similarity). According to PubMed:8666668, it localizes to Golgi apparatus, cytoplasmic vesicle and endoplasmic reticulum. - Information by UniProt
-
参照データベース
- Entrez Gene: 79659 Human
- SwissProt: Q8NCM8 Human
- Unigene: 503721 Human
-
別名
- Cytoplasmic dynein 2 heavy chain 1 antibody
- Cytoplasmic dynein 2 heavy chain antibody
- DYHC2_HUMAN antibody
see all
画像
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab122525 は 3 報の論文で使用されています。
- Toulis V et al. The Deubiquitinating Enzyme Ataxin-3 Regulates Ciliogenesis and Phagocytosis in the Retina. Cell Rep 33:108360 (2020). PubMed: 33176149
- Wang H et al. Expression of dynein, cytoplasmic 2, heavy chain 1 (DHC2) associated with glioblastoma cell resistance to temozolomide. Sci Rep 6:28948 (2016). WB, ICC/IF ; Human . PubMed: 27375225
- Gholkar AA et al. Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis. Cell Cycle 14:1116-25 (2015). PubMed: 25830415