製品の概要

  • 製品名Anti-Dynamin 1 antibody [EP801Y]
    Dynamin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP801Y] to Dynamin 1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus of human dynamin-1.

  • ポジティブ・コントロール
    • 3T3 cell lysate, human brain tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Anti-Dynamin 1 antibody (Alexa Fluor® 488) [EP801Y] (ab206051)

    Anti-Dynamin 1 antibody [EP801Y] (ab52611)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/300.

For unpurified, use 1/50.

WB 1/2000. Predicted molecular weight: 97 kDa.
IHC-P 1/50.

ターゲット情報

Anti-Dynamin 1 antibody [EP801Y] 画像

  • All lanes : Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (purified)

    Lane 1 : C6 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/10000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of Neuro-2a cells with purified ab52611 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52611 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
  • Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-Dynamin 1 antibody [EP801Y] (ab52611) at 1/2000 dilution (unpurified) + 3T3 cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Unpurified ab52611 at 1/100-1/250 dilution staining human brain tissue; paraffin embedded.

    Ab52611 at 1/100-1/250 dilution staining human brain tissue; paraffin embedded.

Anti-Dynamin 1 antibody [EP801Y] (ab52611) 使用論文

This product has been referenced in:
  • Tian X  et al. Podocyte-associated talin1 is critical for glomerular filtration barrier maintenance. J Clin Invest 124:1098-113 (2014). Mouse . Read more (PubMed: 24531545) »
  • Zhang YQ & Chandra SS Oligomerization of Cysteine String Protein alpha mutants causing adult neuronal ceroid lipofuscinosis. Biochim Biophys Acta 1842:2136-2146 (2014). Read more (PubMed: 25064588) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"