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RabMAb

Anti-Dux4 抗体 [E5-5] (ab124699)

製品の概要

  • 製品名
    Anti-Dux4 antibody [E5-5]
    Dux4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E5-5] to Dux4
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    .

  • ポジティブ・コントロール
    • WB: Human, mouse and rat testis and mouse and rat brain tissue lysates. HT 1080, PC 3, and HeLa cells lysates.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    E5-5
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 関連性
      DUX4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in DUX4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Severity is highly variable. Weakness is slowly progressive and about 20% of affected individuals eventually require a wheelchair. Approximately 70-90% of individuals have inherited the disease-causing deletion from a parent, and approximately 10-30% of affected individuals have FSHD as the result of a de novo deletion. Offsprings of an affected individual have a 50% chance of inheriting the deletion.
    • 細胞内局在
      Nuclear
    • 参照データベース
    • 別名
      • Double homeobox 4 antibody
      • Double homeobox protein 10 antibody
      • Double homeobox protein 4 antibody
      • Double homeobox protein 4/10 antibody
      • DUX10 antibody
      • DUX4L antibody
      • DUX4L1 antibody
      see all

    画像

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate
      Lane 3 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)

      Lane 1 : HT-1080 whole cell lysate
      Lane 2 : PC-3 whole cell lysate
      Lane 3 : Saos-2 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse testis tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa


      Exposure time : 3 minutes
    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (unpurified)

      Lane 1 : Human testis lysate
      Lane 2 : HT 1080 cell lysate
      Lane 3 : PC 3 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 45 kDa

    プロトコール

    参考文献

    This product has been referenced in:
    • Homma S  et al. Nuclear bodies reorganize during myogenesis in vitro and are differentially disrupted by expression of FSHD-associated DUX4. Skelet Muscle 6:42 (2016). ICC/IF ; Human . Read more (PubMed: 27906075) »
    • Choi SH  et al. DUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes. Nucleic Acids Res 44:5161-73 (2016). IP . Read more (PubMed: 26951377) »

    See all 7 Publications for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Rhabdomyosarcoma cell line)
    Specification
    Rhabdomyosarcoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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