製品の概要

  • 製品名Anti-DUSP6 antibody
    DUSP6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to DUSP6
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-382 of Human DUSP6a

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab54940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Inactivates MAP kinases. Has a specificity for the ERK family.
  • 配列類似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dual specificity phosphatase 6 antibody
    • Dual specificity phosphatase 6 isoform a antibody
    • Dual specificity protein phosphatase 6 antibody
    • Dual specificity protein phosphatase PYST1 antibody
    • DUS6_HUMAN antibody
    • DUSP 6 antibody
    • DUSP 6a antibody
    • Dusp6 antibody
    • DUSP6a antibody
    • HH19 antibody
    • MAP kinase phosphatase 3 antibody
    • Mitogen activated protein kinase phosphatase 3 antibody
    • Mitogen-activated protein kinase phosphatase 3 antibody
    • MKP 3 antibody
    • MKP-3 antibody
    • MKP3 antibody
    • PYST 1 antibody
    • PYST1 antibody
    • Serine/threonine specific protein phosphatase antibody
    see all

Anti-DUSP6 antibody 画像

  • DUSP6a antibody (ab54940) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human pancreas.


  • Predicted band size : 42 kDa
    DUSP6a antibody (ab54940) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • ab54940 at 10 ug/ml staining DUSP6 in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing SH-SY5Y cells stained with ab54940 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54940, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-DUSP6 antibody (ab54940) 使用論文

This product has been referenced in:
  • Ackley A  et al. An Algorithm for Generating Small RNAs Capable of Epigenetically Modulating Transcriptional Gene Silencing and Activation in Human Cells. Mol Ther Nucleic Acids 2:e104 (2013). WB ; Human . Read more (PubMed: 23839098) »
  • Oliveras-Ferraros C  et al. Cross-suppression of EGFR ligands amphiregulin and epiregulin and de-repression of FGFR3 signalling contribute to cetuximab resistance in wild-type KRAS tumour cells. Br J Cancer 106:1406-14 (2012). Read more (PubMed: 22491422) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab54940.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"