製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to DUSP5
  • アプリケーション
    適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: LKEAFDYIKQ RRSMVSPNFG FMGQLLQYES EILPSTPNPQ PPSCQGEAAG SSLIGHLQTL SPDMQGAYCT FPASVLAPVP THSTVSELSR SPVATATSC, corresponding to amino acids 286-385 of Human DUSP5

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab54939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Displays phosphatase activity toward several substrates. The highest relative activity is toward ERK1.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dual specificity phosphatase 5 antibody
    • Dual specificity protein phosphatase 5 antibody
    • Dual specificity protein phosphatase hVH 3 antibody
    • Dual specificity protein phosphatase hVH3 antibody
    • DUS5_HUMAN antibody
    • DUSP 5 antibody
    • DUSP antibody
    • DUSP5 antibody
    • HVH 3 antibody
    • HVH3 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH 3 antibody
    • VH1 like phosphatase 3 antibody
    • VH3 antibody
    see all

画像



  • Predicted band size : 42 kDa
    DUSP5 antibody (ab54939) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • ab54939 at 10 ug/ml staining DUSP5 in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing SH-SY5Y cells stained with ab54939 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54939, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

参考文献

This product has been referenced in:
  • Wang J  et al. Long Non-coding RNA H19 Induces Cerebral Ischemia Reperfusion Injury via Activation of Autophagy. Aging Dis 8:71-84 (2017). WB . Read more (PubMed: 28203482) »
  • Wickramasekera NT  et al. Role of dual-specificity protein phosphatase-5 in modulating the myogenic response in rat cerebral arteries. J Appl Physiol 114:252-61 (2013). Read more (PubMed: 23172031) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (Hela and K562)
Specification
Hela and K562
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (NSCLC lung cancer cell - A549)
Loading amount
100 µg
Specification
NSCLC lung cancer cell - A549
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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