製品の概要

  • 製品名Anti-DUSP4 antibody
    DUSP4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DUSP4
  • アプリケーション適用あり: ICC/IF, IHC-Fr, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from internal sequence of human DUSP4.

  • ポジティブ・コントロール
    • Paraffin embedded Human brain tissue. Extracts from RAW264.7 cells, A549 cells, COS-7 cells and NIH-3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 23223570
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.
  • 配列類似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • 翻訳後修飾Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dual specificity phosphatase 4 antibody
    • Dual specificity protein phosphatase 4 antibody
    • Dual specificity protein phosphatase hVH2 antibody
    • DUS4_HUMAN antibody
    • DUSP 4 antibody
    • Dusp4 antibody
    • HVH 2 antibody
    • HVH2 antibody
    • MAP kinase phosphatase 2 antibody
    • Mitogen activated protein kinase phosphatase 2 antibody
    • Mitogen-activated protein kinase phosphatase 2 antibody
    • MKP 2 antibody
    • MKP-2 antibody
    • MKP2 antibody
    • Serine/threonine specific protein phosphatase antibody
    • TYP antibody
    • Typ1 antibody
    • VH 2 antibody
    • VH1 homologous phosphatase 2 antibody
    • VH2 antibody
    see all

Anti-DUSP4 antibody 画像

  • All lanes : Anti-DUSP4 antibody (ab72593) at 1/500 dilution

    Lane 1 : Extracts from RAW264.7 cells
    Lane 2 : Extracts from A549 cells
    Lane 3 : Extracts from COS-7 cells
    Lane 4 : Extracts from NIH-3T3 cells
    Lane 5 : Extracts from NIH-3T3 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab72593, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-DUSP4 antibody (ab72593) 使用論文

This product has been referenced in:
  • Sim J  et al. Immunohistochemical expression of dual-specificity protein phosphatase 4 in patients with colorectal adenocarcinoma. Gastroenterol Res Pract 2015:283764 (2015). IHC-P ; Human . Read more (PubMed: 25688264) »
  • Jeong MW  et al. Mitogen-activated protein kinase phosphatase 2 regulates histone H3 phosphorylation via interaction with vaccinia-related kinase 1. Mol Biol Cell 24:373-84 (2013). WB, ICC/IF ; Human . Read more (PubMed: 23223570) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Melanoma cell line xenograft)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization No
Specification Melanoma cell line xenograft
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 23 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (aortic root(heart))
Specification aortic root(heart)
Permeabilization No
Fixative Formaldehyde
Username

Ms. B Ren

Verified customer

投稿 Feb 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"