製品の概要

  • 製品名Anti-DUSP27 antibody
  • 製品の詳細
    Rabbit polyclonal to DUSP27
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 250-282 (EALMTVRKKR AIYPNEGFLK QLRELNEKLM EER) of Human DUSP27 (Q5VZP5).

  • ポジティブ・コントロール
    • Human testis tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab121372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-DUSP27 antibody 画像

  • ab121372 at 1/100 dilution staining DUSP27 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.

Anti-DUSP27 antibody (ab121372) 使用論文

ab121372 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 60 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (mouse muscle C2C12)
Specification mouse muscle C2C12
Treatment siRNA transfected
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Abcam user community

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投稿 Sep 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A
Sample Mouse Cell lysate - whole cell (Kidney)
Specification Kidney
Treatment plasmid transfected
Total protein in input 500 µg
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Abcam user community

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投稿 Sep 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
Sample Mouse Cell (mouse muscle C2C12)
Specification mouse muscle C2C12
Permeabilization Yes - 0.3% triton in PBS
Fixative Paraformaldehyde
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Yanfeng

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投稿 Sep 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"