製品の概要

  • 製品名Anti-DUSP22 antibody
    DUSP22 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DUSP22
  • 特異性ab70124 detects endogenous levels of total DUSP22 protein.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human DUSP22.

  • ポジティブ・コントロール
    • Jurkat and RAW264.7 cell extracts.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ELISA 1/5000.

ターゲット情報

  • 機能Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK).
  • 組織特異性Ubiquitous. Highest expression seen in heart, placenta, lung, liver, kidney and pancreas.
  • 配列類似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dual specificity protein phosphatase 22 antibody
    • DUS22_HUMAN antibody
    • Dusp22 antibody
    • JNK stimulatory phosphatase 1 antibody
    • JNK-stimulatory phosphatase-1 antibody
    • JSP 1 antibody
    • JSP-1 antibody
    • JSP1 antibody
    • LMW DSP2 antibody
    • LMW-DSP2 antibody
    • Low molecular weight dual specificity phosphatase 2 antibody
    • MAP kinase phosphatase x antibody
    • Mitogen activated protein kinase phosphatase x antibody
    • Mitogen-activated protein kinase phosphatase x antibody
    • MKP-x antibody
    • MKPX antibody
    see all

Anti-DUSP22 antibody 画像

  • All lanes : Anti-DUSP22 antibody (ab70124) at 1/500 dilution

    Lane 1 : Jurkat cell extracts
    Lane 2 : RAW264.7 cell extracts
    Lane 3 : RAW264.7 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • ICC/IF image of ab70124 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70124, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DUSP22 antibody (ab70124) 使用論文

ab70124 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"