Anti-DUSP22 抗体 (ab70124)
Key features and details
- Rabbit polyclonal to DUSP22
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DUSP22 antibody
DUSP22 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DUSP22 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide derived from an internal sequence of human DUSP22.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab70124 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70124の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
ターゲット情報
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機能
Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK). -
組織特異性
Ubiquitous. Highest expression seen in heart, placenta, lung, liver, kidney and pancreas. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 100134291 Human
- Entrez Gene: 56940 Human
- Entrez Gene: 105352 Mouse
- SwissProt: Q9NRW4 Human
- SwissProt: Q99N11 Mouse
- Unigene: 29106 Human
- Unigene: 289646 Mouse
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別名
- Dual specificity protein phosphatase 22 antibody
- DUS22_HUMAN antibody
- Dusp22 antibody
see all
画像
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All lanes : Anti-DUSP22 antibody (ab70124) at 1/500 dilution
Lane 1 : Jurkat cell extracts
Lane 2 : RAW264.7 cell extracts
Lane 3 : RAW264.7 cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa -
ICC/IF image of ab70124 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70124, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab70124 は 1 報の論文で使用されています。
- Feng Z et al. Identification of a rhodanine derivative BML-260 as a potent stimulator of UCP1 expression. Theranostics 9:3501-3514 (2019). PubMed: 31281493