製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DUSP10
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 432 - 482 of Human DUSP10 (NP_009138.1)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 53 kDa.
IP Use at 10-15 µg/mg of lysate.

ターゲット情報

  • 機能
    Involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dual specificity phosphatase 10 antibody
    • Dual specificity phosphatase MKP 5 antibody
    • Dual specificity phosphatase MKP5 antibody
    • Dual specificity protein phosphatase 10 antibody
    • DUS10_HUMAN antibody
    • DUSP 10 antibody
    • DUSP10 antibody
    • DUSP10 protein antibody
    • MAP kinase phosphatase 5 antibody
    • Mitogen activated protein kinase phosphatase 5 antibody
    • Mitogen-activated protein kinase phosphatase 5 antibody
    • MKP 5 antibody
    • MKP-5 antibody
    • MKP5 antibody
    • Serine/threonine specific protein phosphatase antibody
    see all

画像

  • All lanes : Anti-DUSP10 antibody (ab87842) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 53 kDa


    Exposure time : 3 minutes
  • ab87842 (lane 1) or a control IgG (lane 2) were used at a concentration of 10µg per mg of lysate to immunoprecipitate 1 mg of HeLa whole cell lysate.
    20% of IP was loaded in each lanel and probed with ab87842 at 1µg/ml.
    Detection: chemiluminescence with exposure time of 30 seconds.

参考文献

ab87842 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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