ab87842 (lane 1) or a control IgG (lane 2) were used at a concentration of 10µg per mg of lysate to immunoprecipitate 1 mg of HeLa whole cell lysate. 20% of IP was loaded in each lanel and probed with ab87842 at 1µg/ml. Detection: chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-DUSP10 antibody (ab87842) at 1 µg/ml
Lane 1 : HeLa whole cell lysate immunoprecipiated with ab to an upstream epitope of DUSP10 Lane 2 : HeLa whole cell lysate immunoprecipitated with ab87842 Lane 3 : HeLa whole cell lystae immunoprecipitated with control IgG