製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DUSP1
  • 特異性
    Targets N terminus of DUSP1.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids within residues 1-100 of DUSP1 (Human)

  • ポジティブ・コントロール
    • HEK 293 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab37493 was eluted out with both high and low pH buffers and neutralised immediately after elution, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

ターゲット情報

  • 機能
    Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
  • 組織特異性
    Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CL 100 antibody
    • CL100 antibody
    • Dual Specificity Phosphatase 1 antibody
    • Dual specificity protein phosphatase 1 antibody
    • Dual specificity protein phosphatase hVH1 antibody
    • DUS1_HUMAN antibody
    • DUSP 1 antibody
    • Dusp1 antibody
    • HVH1 antibody
    • MAP kinase phosphatase 1 antibody
    • Mitogen-activated protein kinase phosphatase 1 antibody
    • MKP-1 antibody
    • MKP1 antibody
    • Protein tyrosine phosphatase CL100 antibody
    • Protein-tyrosine phosphatase CL100 antibody
    • PTPN10 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH1 antibody
    see all

画像

  • Anti-DUSP1 antibody (ab37493) + HEK 293 cell lysate.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 16 kDa,22 kDa,75 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab37493 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry. The optimal conditions for this antibody fall within these guidelines: 1) At an antibody concentration of 2ug/ml, the amount of protein loaded should be ~10ug 2. The blocking reagent: 3% milk in TBST 3. The...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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