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Our Abpromise guarantee covers the use of ab1351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml.|
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Most of the commercially available BSA and dry milk are contaminated with bovine IgG (even some of the higher purity grades of BSA); this might cause high background problems when labelled anti-goat secondary is used. The secondary might cross-react with bovine IgG since goat is closely related to cow.