Anti-MKP-1 抗体 (ab1351)
Key features and details
- Goat polyclonal to MKP-1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MKP-1 antibody
MKP-1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MKP-1 -
由来種
Goat -
特異性
The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Dog -
免疫原
Synthetic peptide corresponding to Human MKP-1 aa 355-367 (C terminal). The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).
Sequence:SYLQSPITTSPSC
Database link: P28562 -
ポジティブ・コントロール
- Recombinant Human MKP-1 protein (ab114331) can be used as a positive control in WB. WB: HeLa cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1351の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (5) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 39 kDa.
A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
特記事項 |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 39 kDa. A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
ターゲット情報
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機能
Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'. -
組織特異性
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 rhodanese domain.
Contains 1 tyrosine-protein phosphatase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 1843 Human
- Omim: 600714 Human
- SwissProt: P28562 Human
- Unigene: 171695 Human
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別名
- CL 100 antibody
- CL100 antibody
- Dual Specificity Phosphatase 1 antibody
see all
画像
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Anti-MKP-1 antibody (ab1351) at 1 µg/ml + HeLa cell lysate at 35 µg
Predicted band size: 39 kDa
Observed band size: ~40 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (9)
ab1351 は 9 報の論文で使用されています。
- Xin Y et al. Inhibition of miR-101-3p protects against sepsis-induced myocardial injury by inhibiting MAPK and NF-?B pathway activation via the upregulation of DUSP1. Int J Mol Med 47:N/A (2021). PubMed: 33448324
- Dedobbeleer M et al. MKP1 phosphatase is recruited by CXCL12 in glioblastoma cells and plays a role in DNA strand breaks repair. Carcinogenesis 41:417-429 (2020). PubMed: 31504251
- Fu XH et al. Dual-specificity phosphatase 1 regulates cell cycle progression and apoptosis in cumulus cells by affecting mitochondrial function, oxidative stress, and autophagy. Am J Physiol Cell Physiol 317:C1183-C1193 (2019). PubMed: 31532716
- Unni AM et al. Hyperactivation of ERK by multiple mechanisms is toxic to RTK-RAS mutation-driven lung adenocarcinoma cells. Elife 7:N/A (2018). PubMed: 30475204
- Jin Q et al. Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway. Mol Med Rep 16:1472-1478 (2017). PubMed: 29067454
- Shen J et al. DUSP1 inhibits cell proliferation, metastasis and invasion and angiogenesis in gallbladder cancer. Oncotarget 8:12133-12144 (2017). PubMed: 28129656
- Delehouzé C et al. CDK/CK1 inhibitors roscovitine and CR8 downregulate amplified MYCN in neuroblastoma cells. Oncogene N/A:N/A (2013). Human . PubMed: 24317512
- Giachini FR et al. Extracellular signal-regulated kinase 1/2 activation, via downregulation of mitogen-activated protein kinase phosphatase 1, mediates sex differences in desoxycorticosterone acetate-salt hypertension vascular reactivity. Hypertension 55:172-9 (2010). WB ; Rat . PubMed: 19901158
- Nguyen KA et al. Modulation of gonadotropin-releasing hormone-induced extracellular signal-regulated kinase activation by dual-specificity protein phosphatase 1 in LbetaT2 gonadotropes. Endocrinology 151:4882-93 (2010). WB ; Mouse . PubMed: 20685880