製品の概要

  • 製品名Anti-DTX3L antibody
    DTX3L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DTX3L
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 700 and the C-terminus (residue 740) of human DTX3L (NP_612144.1)

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the Deltex family.
    Contains 1 RING-type zinc finger.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B lymphoma and BAL associated protein antibody
    • B-lymphoma- and BAL-associated protein antibody
    • BBAP antibody
    • Deltex 3 like antibody
    • DTX 3L antibody
    • DTX3L antibody
    • DTX3L_HUMAN antibody
    • E3 ubiquitin-protein ligase DTX3L antibody
    • Protein deltex 3 like antibody
    • Protein deltex 3 like protein antibody
    • Protein deltex-3-like antibody
    • Rhysin 2 antibody
    • Rhysin-2 antibody
    • Rhysin2 antibody
    see all

Anti-DTX3L antibody 画像

  • All lanes : Anti-DTX3L antibody (ab70621) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 83 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,40 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70621 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab70621 at 1 µg/ml.

Anti-DTX3L antibody (ab70621) 使用論文

ab70621 has not yet been referenced specifically in any publications.

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