Anti-DTX3L 抗体 (ab70621)
Key features and details
- Rabbit polyclonal to DTX3L
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DTX3L antibody
DTX3L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DTX3L -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human DTX3L aa 700 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8TDB6 -
ポジティブ・コントロール
- WB: HeLa, HEK-239T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70621の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the Deltex family.
Contains 1 RING-type zinc finger. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 151636 Human
- Entrez Gene: 209200 Mouse
- Omim: 613143 Human
- SwissProt: Q8TDB6 Human
- SwissProt: Q3UIR3 Mouse
- Unigene: 518201 Human
- Unigene: 390852 Mouse
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別名
- B lymphoma and BAL associated protein antibody
- B-lymphoma- and BAL-associated protein antibody
- BBAP antibody
see all
画像
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All lanes : Anti-DTX3L antibody (ab70621) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 40 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70621 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab70621 at 1 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab70621 は論文での使用が確認できていません。