製品の概要

  • 製品名Anti-DSCC1 antibody
    DSCC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DSCC1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region within C terminal amino acids 318-347 of Human DSCC1 (NP_076999.2).

  • ポジティブ・コントロール
    • CEM cell line lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 45 kDa.
ICC/IF 1/200. Use with paraformaldehyde-fixed cells.

ターゲット情報

  • 機能Loads PCNA onto primed templates regulating velocity, spacing and restart activity of replication forks. May couple DNA replication to sister chromatid cohesion through regulation of the acetylation of the cohesin subunit SMC3.
  • 配列類似性Belongs to the DCC1 family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DCC1 antibody
    • DCC1_HUMAN antibody
    • Defective in sister chromatid cohesion 1 homolog antibody
    • Defective in sister chromatid cohesion protein 1 homolog antibody
    • DSCC1 antibody
    • hDCC1 antibody
    • MGC5528 antibody
    • Sister chromatid cohesion protein DCC1 antibody
    see all

Anti-DSCC1 antibody 画像

  • Anti-DSCC1 antibody (ab106972) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 45 kDa
  • ab106972 (1/200) staining DSCC1 in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details, please refer to Abreview.

    See Abreview

Anti-DSCC1 antibody (ab106972) 使用論文

ab106972 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 90 µg
Gel Running Conditions Reduced Denaturing (4-18% gel)
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification Mouse embryonic fibroblasts
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

投稿 Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"