製品の概要

  • 製品名Anti-Dscam antibody
    Dscam 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dscam
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 1547-1596 (

    MRVCNSAGCAEKQANFATLNYDGSTIPPLIKSVVQNEEGLTTNEGLKMLV

    ) of Human Dscam (NP_001380).

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 222 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

ターゲット情報

  • 機能Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38.
  • 組織特異性Primarily expressed in brain.
  • 配列類似性Contains 6 fibronectin type-III domains.
    Contains 10 Ig-like C2-type (immunoglobulin-like) domains.
  • ドメインIg-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC.
  • 翻訳後修飾Phosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin.
  • 細胞内局在Secreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CHD2 42 antibody
    • CHD2 52 antibody
    • CHD2 antibody
    • Down syndrome cell adhesion molecule antibody
    • DSCAM antibody
    • DSCAM_HUMAN antibody
    • human CHD2-52 down syndrome cell adhesion molecule 2, 9 antibody
    • OTTHUMP00000109193 2 antibody
    • SD11109p antibody
    see all

Anti-Dscam antibody 画像

  • Anti-Dscam antibody (ab85362) at 1 µg/ml + 10 µg HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50,000.

    Predicted band size : 222 kDa

Anti-Dscam antibody (ab85362) 使用論文

ab85362 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (embryonic brain tissue, stage 10.5)
Specification embryonic brain tissue, stage 10.5
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Tween
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Username

Dr. Minkyung Kim

Verified customer

投稿 Jun 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"