Anti-Dscam 抗体 (ab51974)
Key features and details
- Goat polyclonal to Dscam
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Dscam antibody
Dscam 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Dscam -
由来種
Goat -
特異性
This antibody is expected to recognise both reported isoforms. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Dog -
免疫原
Peptide with sequence CDSWDSAQRTKDVSPQ, from the internal region of the protein sequence (Human)
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ポジティブ・コントロール
- NIH/3T3 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51974の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 170 kDa.
A 1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 170 kDa. A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. -
組織特異性
Primarily expressed in brain. -
配列類似性
Contains 6 fibronectin type-III domains.
Contains 10 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
Ig-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC. -
翻訳後修飾
Phosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin. -
細胞内局在
Secreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons. - Information by UniProt
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参照データベース
- Entrez Gene: 1826 Human
- Entrez Gene: 13508 Mouse
- Entrez Gene: 171119 Rat
- Omim: 602523 Human
- SwissProt: O60469 Human
- SwissProt: Q9ERC8 Mouse
- SwissProt: Q8VHZ8 Rat
- Unigene: 397800 Human
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別名
- CHD2 42 antibody
- CHD2 52 antibody
- CHD2 antibody
see all
画像
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Anti-Dscam antibody (ab51974) at 1 µg/ml + NIH/3T3 cell lysate in RIPA buffer at 35 µg
Observed band size: 200 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Anti-Dscam antibody (ab51974) at 2 µg/ml + Human Brain (Cerebellum) lysate in RIPA buffer at 35 µg
Observed band size: 170 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab51974 は論文での使用が確認できていません。