製品の概要

  • 製品名Anti-ds DNA antibody [35I9 DNA]
    ds DNA 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [35I9 DNA] to ds DNA
  • 特異性Primarily Double stranded DNA. Measurements by immuno-CE yielded KD's of 0.71 µM and 0.09 µM, for the interaction of this antibody with ss- and dsDNA, respectively. Strong reactivity with both ss- and dsDNA has been observed on dotblots as well as very weak reactivity with RNA.
  • アプリケーション適用あり: IHC-Fr, Dot blot, ICC, ICC/IF, ELISAmore details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 特記事項

    This antibody has been shown to be useful, using immunofluorescence, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis as well as ELISA-based co-culture angiogenesis and proliferation assays. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 一次抗体 備考This antibody has been shown to be useful, using immunofluorescence, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis as well as ELISA-based co-culture angiogenesis and proliferation assays. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength.
  • ポリ/モノモノクローナル
  • クローン名35I9 DNA
  • ミエローマx63-Ag8.653
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
Dot blot 1/1000.
ICC Use at an assay dependent concentration.
ICC/IF 1/1000.
ELISA 1/2000.

ターゲット情報

  • 関連性dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
  • 細胞内局在Nuclear
  • 別名
    • Double stranded deoxyribonucleic acid antibody
    • Double stranded DNA antibody
    • dsDNA antibody

Anti-ds DNA antibody [35I9 DNA] 画像

  • ab27156 at 1/1000 staining Stylonychia lemnae cells (isolated macronucleus anlagen) by ICC. The cells were methanol fixed and incubated with the antibody for 1 hour. A Cy3 ® conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

Anti-ds DNA antibody [35I9 DNA] (ab27156) 使用論文

This product has been referenced in:
  • Bates MA  et al. Silica-Triggered Autoimmunity in Lupus-Prone Mice Blocked by Docosahexaenoic Acid Consumption. PLoS One 11:e0160622 (2016). ELISA ; Mouse . Read more (PubMed: 27513935) »
  • Limoli DH  et al. Cationic antimicrobial peptides promote microbial mutagenesis and pathoadaptation in chronic infections. PLoS Pathog 10:e1004083 (2014). Read more (PubMed: 24763694) »

See all 11 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (whole mount larval brain)
Permeabilization Yes - Triton 0.3%
Specification whole mount larval brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 02 2016

Application Immunoprecipitation
Sample Human Cell lysate - other (NA)
Total protein in input 0 µg
Immuno-precipitation step Other - dynabeads protein G
Specification NA
Username

Abcam user community

Verified customer

投稿 Apr 15 2015


ab27156 was validated in ELISA, coated with 20 ug/ml dsDNA in PBS. The antibody should be diluted from 0.5 to 2 µg/ml, depending on the batch.

Thank you for contacting us.

Please check the following primary antibody datasheets

http://www.abcam.com/ds-DNA-antibody-HYB331-01-ab27156.html

http://www.abcam.com/ds-DNA-antibody-11B6-ab418.html

The compatible seco...

Read More

Thank you for your inquiry and I appreciate your patience with this reply.
For ab27156,the lab did nothave any data on how much DNA can be detected with the ds DNA antibody. However different specificities regarding the binding characteristics of ...

Read More

Thank you for contacting us. Please check the datasheet for anti DNA antibodies ab27156; http://www.abcam.com/ds-DNA-antibody-HYB331-01-ab27156.html ab418; http://www.abcam.com/ds-DNA-antibody-11B6-ab418.html Please check the compatible s...

Read More

Thank you for contacting us. I apologize for being late in getting back to you as it took longer than anticipated. I can confirm that the antibody does not have any sodium azide in it. It was filtered sterile. Our lab has also reported that they...

Read More

Thank you for contacting us. To answer you questions regarding ab27156: 1) Unfortunately, the details of the immunogen for this antibody are not available. Therefore, I do not know where the antibody binds and what the epitope would be. ...

Read More

Thank you for contacting us. I can confirm that I have logged your inquiry and have contacted lab for the information you requested. I will get back to you with the information soon. I hope this information is helpful to you. Please do n...

Read More

Thank you for contacting us. I am happy to confirm that the laboratory answered that this antibody recognizes dsDNA independently of the sequence. Although the laboratory has not tested specifically the poly dA-dT composition, we will cover this ...

Read More

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"