Application
Immunoprecipitation
Sample
Human Cell lysate - other (NA)
Total protein in input
0 µg
Immuno-precipitation step
Other - dynabeads protein G
Specification
NA
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Western Blot antibody
Western Blot antibody
None used
Additional data
Additional Notes
Pulldown of DNA using anti-ds DNA antibody
experimental details of pulldown experiment for 100 bp PCR fragment:
5 pmol of radioactive labeled PCR fragment was incubated with different amount of anti-ds DNA antibody (1 ug, 2 ug, 4 ug) for 2 hours at room temperature, then immobilized on Dynabeads Protein G (12 hours, 4 °C).
Buffer: PBS + 0.5% BSA + 0.1% Tween20
Result: 4 ug of antibody pulled-down 90% of 5 pmol DNA.
Abcam response
Please note, this IP experient has been done under Abtrial.
As the precipitated material 100bp PCR product was used and NOT a cell lysate (please read the customer's comments which describe the experiment).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 15 2015