製品の概要
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製品名Anti-DRIM antibody
DRIM 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to DRIM
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由来種Rabbit
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アプリケーション適用あり: WB, IPmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Horse, Cow, Chimpanzee, Rhesus monkey, Bat, Elephant
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免疫原
Synthetic peptide, corresponding to a region between residues 2735 and 2785 of human DRIM (NP_055318.2)
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ポジティブ･コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab86093 was affinity purified using an epitope specific to DRIM immobilized on solid support.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab86093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 318 kDa (predicted molecular weight: 318 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性DRIM, also known as UTP20, is a component of the U3 small nucleolar RNA (snoRNA) protein complex (U3 snoRNP) and is involved in 18S rRNA processing. DRIM contains 2 HEAT repeats.
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細胞内局在Nucleus; nucleolus
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参照データベース
- Entrez Gene: 27340 Human
- Entrez Gene: 70683 Mouse
- Omim: 612822 Human
- SwissProt: O75691 Human
- SwissProt: Q5XG71 Mouse
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別名
- 3830408P06Rik antibody
- AA617408 antibody
- Down regulated in metastasis antibody
see all
画像
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Western blot - DRIM antibody (ab86093)All lanes : Anti-DRIM antibody (ab86093) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 318 kDa
Observed band size: 318 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunoprecipitation - DRIM antibody (ab86093)Detection of DRIM by Western Blot of Immunprecipitate.
ab86093 at 1µg/ml staining DRIM in HeLa whole cell lysate immunoprecipitated using ab86093 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
参考文献
ab86093 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"