This antibody gave a positive signal in the following lysates:
HeLa Whole Cell; HeLa Nuclear; Jurkat Whole Cell; Jurkat Nuclear; HepG2 Whole Cell; HepG2 Nuclear (Data not shown for HepG2 Whole Cell and HepG2 Nuclear lysates).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
Use a concentration of 1 µg/ml.
Binds to 5'-TGTCG[CT]GA[CT]A-3' DNA elements found in the promoter regions of a number of genes related to cell proliferation. Binds to the histone H1 promoter and stimulates transcription. Was first identified as gene weakly similar to Ac transposable elements, but does not code for any transposase activity.
Ubiquitously expressed at low levels. Expression is highest in skeletal muscle, heart, spleen and placenta.
BED type zinc finger domain containing protein 1 antibody
DNA replication related element binding factor antibody
dREF homolog antibody
Putative Ac like transposon antibody
Putative Ac-like transposable element antibody
Zinc finger BED domain-containing protein 1 antibody
Zinc finger BED type containing 1 antibody
Western blot - DREF antibody (ab48355)
All lanes : Anti-DREF antibody (ab48355) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 4 :Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 78 kDa Observed band size: 78 kDa Additional bands at: 27 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - DREF antibody (ab48355)
ICC/IF image of ab48355 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab48355, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).