製品の概要

  • 製品名Anti-Drebrin antibody [M2F6]
    Drebrin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [M2F6] to Drebrin
  • 特異性This antibody detects Drebrin A and E in samples from human, mouse, rat, guinea pig, and cat origins and Drebrin E only in chicken samples.
  • アプリケーション適用あり: WB, IHC-P, IHC-Fr, ICC, ICC/IF, IHC-FoFr, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Guinea pig, Cat, Monkey, Quail
  • 免疫原

    Chicken Drebrin E protein.

  • ポジティブ・コントロール
    • Mouse Brain Tissue Extract (ab7934).
  • 特記事項For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 10 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 65 kDa). The difference in the size of the detected band (~110 kDa) and in the predicted (~65 kDa) is due to the large number of negatively charged residues in this protein. Block membrane with 5-10% milk for 1 hour.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration. Fix cells with 4% PFA for 15 minutes. Permeabilise cells for 15 minutes with 0.1% triton X100 in PBS (see Ikeda K et al).
ICC/IF Use at an assay dependent concentration. See Toda et al.
IHC-FoFr 1/1000. See Keon et al.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

Anti-Drebrin antibody [M2F6] 画像

  • IHC-P image of Drebrin staining on Human ovarian carcinoma sections using ab12350 (1:100). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab12350 was diluted 1:100 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab12350 for 16 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).

    See Abreview

  • ab12350 at 1/50 staining mouse brain tissue sections by IHC-P. The tissue fixed in 4% Formalin, diluted in Dulbecco A PBS with standard pwax processing. Citric acid 0.01M pH6 was used as antigen retrieval solution. The tissue sections were incubated with the antibody for 2 hours. A biotinylated goat polyclonal antibody was used as the secondary. The image shows the lateral ventricle showing high cytoplasmic positivity in migrating progenitor neurones in the subventricular space.There is a low level of positivity in the dendritic field of the striatum; this appears to be specific. An identical pattern of staining is seen in same area of rat brain. All non-migratory cells in the same field are negative. The Corpus Callosum is negative.

    See Abreview

  • Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Drebrin antibody [M2F6] (ab12350) on Quail Tissue sections (embryo d5/6 brain). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody: ab12350 incubated at 1/200 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti mouse Igs (1/200).
  • Immunofluorescent staining in the rat brain cerebellum with ab12350 mouse monoclonal Drebrin antibody. Drebrin-positive neurons and fibers are observed. Picture taken with X20 objective. Protocol: IHC free-floating protocol using 4% PFA fixed brain tissue. Rats were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Primary antibody ab12350 was used at 1/1000 incubated overnight at room temperature. Secondary antibody was Alexa Fluor 488 used at 1/1000, 2h incubation at room temperature. Image recoloured in Adobe photoshop.
  • All lanes : Anti-Drebrin antibody [M2F6] (ab12350) at 1/5000 dilution

    Lane 1 : Trangenic (APP/PS1) male mouse cortical lysate
    Lane 2 : Trangenic (APP/PS1) male mouse cortical lysate
    Lane 3 : Trangenic (APP/PS1) male mouse cortical lysate
    Lane 4 : Trangenic (APP/PS1) male mouse cortical lysate
    Lane 5 : Wild-type male mouse cortical lysate
    Lane 6 : Wild-type male mouse cortical lysate
    Lane 7 : Wild-type male mouse cortical lysate
    Lane 8 : Wild-type male mouse cortical lysate
    Lane 9 : Rat cortical lysate

    Secondary
    Alexa-Fluor 680 conjugated goat anti-mouse IgG

    Predicted band size : 65 kDa
    Observed band size : 42,95,120 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Dr Martin Broadstock

    Western blot showing detection of drebrin in mouse and rat cortical lysates by ab12350.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab12350 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12350, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.



  • Predicted band size : 65 kDa
    Western blot analysis of Drebrin expression in mouse brain (1) NIH/3T3 (2), Jurkat (3), HeLa (4), and PC12 (5) using Drebrin mAb (ab12350).

Anti-Drebrin antibody [M2F6] (ab12350) 使用論文

This product has been referenced in:
  • Li L  et al. Chronic intermittent fasting improves cognitive functions and brain structures in mice. PLoS One 8:e66069 (2013). WB ; Mouse . Read more (PubMed: 23755298) »
  • Kreis P  et al. Phosphorylation of the actin binding protein Drebrin at S647 is regulated by neuronal activity and PTEN. PLoS One 8:e71957 (2013). Read more (PubMed: 23940795) »

See all 17 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 03 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Ovarian Carcinoma)
Specification Ovarian Carcinoma
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 May 31 2013

Thank you for your reply and for providing that information.

I have organized for a replacement vial of ab12350 to be send to you. As you purchased the antibody from Cedarlane, we need to send to them first and they will then ship it out to y...

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I am sorry about all the emails, however I do need to make sure that I am sending a replacement to the correct place and since you ordered through Cedarlane, I cannot just look at the shipping address. The PO and order number you provided did not match...

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Thank you for your reply.

I think I have found your original order and so will be able to get you the replacement next week. Before going further, could you confirm the date that the original order was placed and also confirm that you are ok w...

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Thank you for your reply.

I would be happy to send you a different lot of the antibody. Before I can do that though, I do need to have the original order information. Since you ordered via Cedarlane, would you please contact them and ask them ...

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Thank you for your reply and for providing that information.

Based on the information that you have provided, I believe the best way forward is to either replace or refund the antibody for you. Unfortunately we do not have another lot of ab123...

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Thank you for contacting Abcam.

I am sorry about the issues that you are having with the current lot of ab12350 in IHC-P. The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on human samples.

...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT-116)
Loading amount 30 µg
Specification HCT-116
Gel Running Conditions Non-reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Qifeng LIN

Verified customer

投稿 Jan 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Neural stem cell line, Cor-1)
Specification Neural stem cell line, Cor-1
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Trition
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 28 2009

1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"