製品の概要

  • 製品名DRAQ7™ 1ml (0.3mM)
  • アプリケーション適用あり: FM, Flow Cyt, ICC/IFmore details
  • 特記事項

    DRAQ7™ is a new far-red fluorescent dye that only stains the nuclei in dead and permeabilized cells and can be used in combination with common labels such as GFP or FITC. DRAQ7™ is the ideal tool to study dead or membrane-compromised cells because it does not enter intact, live cells. DRAQ7™ is an ideal replacement for propidium iodide (and 7-AAD), having far better spectral properties; it has no UV excitation and no emission overlap with PE and homologues.
    Key features of DRAQ7™ include:

    • Rapid staining of dsDNA/ nuclei of DEAD or permeabilized cells
    • Low photobleaching effect
    • It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc ...
    • Non-toxic in long-term culture
    • Can be combined with "live" dyes
    • No compensation needed with common FITC/GFP + PE combinations in flow cytometry
    • No wash or RNase treatment needed.

    SPECTRAL PROPERTIES

    Excitation:

    • 633 and 647 nm line optimal (Exmax 599 / 644 nm)
    • 488, 514 and 568 nm lines, sub-optimal (only by flow cytometry)

    Emission (instrument dependent):

    • 665 nm to infra-red max 678 nm / 697 nm intercalated with dsDNA)
    • Minimal overlap with vis range e.g. GFP and FITC
    • Em. filters may include 695L, 715LP or 780 LP

    Multi-wavelength imaging with UV / vis fluorochromes

    • No fluorescence enhancement upon DNA binding
    • Low photo-bleaching effect
    • Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluoroscence microscopes

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab109202 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    FM Use at an assay dependent concentration.
    Flow Cyt 1/100. (final concentration = 3µM)
    ICC/IF 1/100. (final concentration = 3µM)
    It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet.

    DRAQ7™ 1ml (0.3mM) 画像

    • Jurkat cells exposed to 1µM staurosporine for 24 hours show DRAQ7™ staining (top half of the plot). These cells have compromised membranes that allow entry of DRAQ7™ in the cells.
    • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
      Preparation:

      • Fix in 3%PFA in PBS for 30 min at RT
      • Incubate in 7.5% sucrose-PBS for 3h at RT
      • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
      • Embed the EBs in tissue-Tek OCT compound
      • Cut frozen sections to 4-20 µm thickness

      Primary antibody: Rabbit anti-laminin alpha 1, 1:400
      Secondary antibody: Goat anti-rabbit IgG - H&L (AMCA) (ab123435)

      Nuclei were counterstained stained with DRAQ7™ (ab109202)

    • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

      Preparation:

      • Fix in 3%PFA in PBS for 30 min at RT
      • Incubate in 7.5% sucrose-PBS for 3h at RT
      • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
      • Embed the EBs in tissue-Tek OCT compound
      • Cut frozen sections to 4-20 µm thickness


      Primary antibody: Rabbit anti-laminin alpha 1, 1:400
      Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1:200
      F-actin was stained with
      CytoPainter F-actin staining kit (blue) (ab112124), 1:1000
      Nuclei were counterstained stained with DRAQ7TM, 1:1000

    プロトコール

    DRAQ7™ 1ml (0.3mM) (ab109202) 使用論文

    This product has been referenced in:
    • Kushner EJ  et al. Excess Centrosomes Perturb Dynamic Endothelial Cell Repolarization During Blood Vessel Formation. Mol Biol Cell : (2016). IHC . Read more (PubMed: 27099371) »
    • Zhou L  et al. Targeting MET and AXL overcomes resistance to sunitinib therapy in renal cell carcinoma. Oncogene 35:2687-97 (2016). Flow Cyt . Read more (PubMed: 26364599) »

    See all 4 Publications for this product

    Product Wall



    Ich habe nun vom Labor eine Antwort erhalten und kopiere diese im Original für Sie:

    "In brief, it is likely that there will be significant washout of the DRAQ7 from the leaky cells into the previously viable ones when perfo...

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    Human colon carcinoma cells stained with DRAQ7

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    The cells were fixed with 4% PFA and permeabilized with 0.5% Triton. They were stained with DRAQ7 diluted 1:100 in PBS.
    Username

    Dr. Eleni Petsalaki

    Verified customer

    投稿 Feb 28 2014



    1) use with tissue samples:
    DRAQ7 should work in this situation but any cell impermeant (viability) dye will have problems penetrating thick sections, in this case perhaps 50+ cell layers thick. A collaborator has used it with micro-tissu...

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    The bottom line is that toxicity studies have yet to been done on DAPI. I have seen one article questioning its use (Qin, 2011 using it on MSCs).
    DAPI is known to be semi-permeant to cells and thus it does get in. As a very high affinity DNA bind...

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    Thank you for your enquiry.

    My colleagues have just uploaded the SDS document so your customer could get access to the requested form by clinking on the hyperlink.

    If you need any further assistance in the future, please do not hesi...

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    Thank you for contacting us.

    We have several products from the CytoPainter range which may be of interest to your customer. These can be accessed from the following link:

    CytoPainter staining kits | Abcam

    These kits allow for...

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    Thank you for your enquiry.

    We recommend Draq5TM, ab108410, for your application, rather than Draq7TM. The laboratory has provided the following notes which describe the differences between the two. In brief, the max emission wavelengths are...

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    Thank you for your patience. I have now received the answer in regards to the use of DRAQ7 on yeast. Unfortunately the laboratory does not know neither whether it will work on yeast cells, due to the difference in the cell wall. I am very sorry there...

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    Thank you for contacting us. Indeed, DRAQ5 has already been used successfully in yeast already. Please see here below the publication citing it: DRAQ5 - http://aem.asm.org/content/72/10/6725.abstract I expect DRAQ7 to be suitable as well for yeast, as...

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    Thank you for your inquiry. DRAQ7 is a LIVE cell impermeant far-red DNA dye. It can be used to identify permeable cells in a mix of live and dead cells (i.e. the dead cells) – but in a preparation of fixed cells, all of the cells will be permeant an...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"