Anti-DRAM 抗体 [M3-P4B4] (ab64739)

製品の概要

  • 製品名
    Anti-DRAM antibody [M3-P4B4]
    DRAM 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [M3-P4B4] to DRAM
  • アプリケーション
    適用あり: WBmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from within residues 150 - 250 of Human DRAM.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa whole cell lysate.
  • 特記事項

    This antibody clone is manufactured by Abcam.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 10 µg/ml. Predicted molecular weight: 26 kDa.
  • 追加情報
    Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能
      Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy.
    • 配列類似性
      Belongs to the DRAM/TMEM150 family.
    • 細胞内局在
      Lysosome membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Damage regulated autophagy modulator antibody
      • Damage-regulated autophagy modulator 1 antibody
      • Damage-regulated autophagy modulator antibody
      • DNA damage-regulated autophagy modulator protein 1 antibody
      • DRAM antibody
      • dram1 antibody
      • DRAM1_HUMAN antibody
      • FLJ11259 antibody
      see all

    画像

    • Anti-DRAM antibody [M3-P4B4] (ab64739) at 10 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 26 kDa
      Observed band size : 26 kDa


      Exposure time : 20 minutes

    プロトコール

    参考文献

    ab64739 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Thank you very much for confirming the details.

    I have checked the history of this antibody and can confirm that we haven't received specificity complaints for this product so I am sure protocol troubleshooting will help in getting a clean wes...

    Read More

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I have read the details you have kindly provided and would like to ask few questions in order to understand the problem better;

    ...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (glioma stem cells)
    Specification
    glioma stem cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton-x 0.1%
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 26 2012

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (HeLa Ms Stomach Ms Kidney Ms Liver)
    Loading amount
    20 µg
    Specification
    HeLa Ms Stomach Ms Kidney Ms Liver
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 May 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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