製品の概要

  • 製品名Anti-DRAK2 antibody [EPR3163Y]
    DRAK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3163Y] to DRAK2
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human DRAK2 aa 250-350.

  • ポジティブ・コントロール
    • Jurkat and Ramos cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 42 kDa.
IP 1/30 - 1/50.
ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能Acts as a positive regulator of apoptosis.
  • 組織特異性Highly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DAP kinase related apoptosis inducing protein antibody
    • DAP kinase related apoptosis inducing protein kinase 2 antibody
    • DAP kinase-related apoptosis-inducing protein kinase 2 antibody
    • Death associated protein kinase related 2 antibody
    • DRAK 2 antibody
    • DRAK2 antibody
    • Serine/threonine kinase 17b antibody
    • Serine/threonine kinase 17b apoptosis inducing antibody
    • Serine/threonine protein kinase 17B antibody
    • Serine/threonine-protein kinase 17B antibody
    • ST17B_HUMAN antibody
    • STK 17B antibody
    • Stk17b antibody
    see all

Anti-DRAK2 antibody [EPR3163Y] 画像



  • Predicted band size : 42 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: DRAK2 knockout HAP1 cell lysate (20 µg)
    Lane 3: Ramos cell lysate (20 µg)
    Lane 4: Raji cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab108373 observed at 42 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab108373 was shown to recognize DRAK2 when DRAK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and DRAK2 knockout samples were subjected to SDS-PAGE. ab108373 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunofluorescence staining of Ramos cells with purified ab108373 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108373 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • All lanes : Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/1000 dilution (purified)

    Lane 1 : C6 whole cell lysate
    Lane 2 : PC-12 whole cell lysate
    Lane 3 : NIH/3T3 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/10000 dilution (purified)

    Lane 1 : Ramos whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • ab108373 (purified) at 1/40 immunoprecipitating DRAK2 in 10 μg Ramos cell lysate (Lanes 1 and 2, observed at 42 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • All lanes : Anti-DRAK2 antibody [EPR3163Y] (ab108373) at 1/500 dilution (Unpurified)

    Lane 1 : Jurkat cell lysate +TPA
    Lane 2 : Ramos cell lysate
    Lane 3 : Ramos cell lysate + TPA

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 42 kDa

Anti-DRAK2 antibody [EPR3163Y] (ab108373) 使用論文

ab108373 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"