The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125-0.25µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
ab101026-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
Squamous cell carcinoma of the head and neck
Contains 1 death domain. Contains 3 TNFR-Cys repeats.
Flow cytometry staining of non-transfected (left) and mouse DR5 transfected (right) BHK cells with 0.125 µg of Golden Syrian Hamster IgG Isotype Control PE (open histogram) or 0.125 µg of ab95619 (filled histogram). Total viable cells were used for analysis.