製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DR5
  • 特異性
    Does not cross react with DR4.
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human DR5 aa 388-407. DR5 antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human DR5 precursor. The immunogen is located within the last 50 amino acids of DR5.
    (Peptide available as ab8449)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/250 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 47.8 kDa).Can be blocked with Human DR5 peptide (ab8449).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
  • 組織特異性
    Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
  • 関連疾患
    Squamous cell carcinoma of the head and neck
  • 配列類似性
    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fas like protein antibody
    • Apoptosis inducing protein TRICK2A/2B antibody
    • Apoptosis inducing receptor TRAIL R2 antibody
    • CD262 antibody
    • CD262 antigen antibody
    • Cytotoxic TRAIL receptor 2 antibody
    • Death domain containing receptor for TRAIL/Apo 2L antibody
    • Death receptor 5 antibody
    • DR5 antibody
    • KILLER antibody
    • KILLER/DR5 antibody
    • OTTHUMP00000123492 antibody
    • OTTHUMP00000123493 antibody
    • p53 regulated DNA damage inducible cell death receptor(killer) antibody
    • TNF related apoptosis inducing ligand receptor 2 antibody
    • TNF-related apoptosis-inducing ligand receptor 2 antibody
    • TNFRSF10B antibody
    • TR10B_HUMAN antibody
    • TRAIL R2 antibody
    • TRAIL receptor 2 antibody
    • TRAIL-R2 antibody
    • TRAILR2 antibody
    • TRICK2 antibody
    • TRICK2A antibody
    • TRICK2B antibody
    • TRICKB antibody
    • Tumor necrosis factor receptor like protein ZTNFR9 antibody
    • Tumor necrosis factor receptor superfamily member 10B antibody
    • Tumor necrosis factor receptor superfamily, member 10b antibody
    • ZTNFR9 antibody
    see all

画像

  • All lanes : Anti-DR5 antibody (ab8416) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : K562 whole cell lysate


    Predicted band size : 47.8 kDa
  • ab8416 at 5µg/ml staining DR5 in Hela cells by ICC/IF.

  • The image above is a xenograph of human cancer cells implanted into a nude mouse and allowed to grow for about three weeks. Notice only the human cancer cells expressed this antigen. This picture was kindly submitted as part of the reveiw by Dean Wilkie.

参考文献

This product has been referenced in:
  • Kuo YJ  et al. Synergistic inhibition of leukemia WEHI-3 cell growth by arsenic trioxide and Hedyotis diffusa Willd extract in vitro and in vivo. Exp Ther Med 13:3388-3396 (2017). Read more (PubMed: 28587418) »
  • Yao Q  et al. Prognostic significance of TRAIL signalling molecules in cervical squamous cell carcinoma. J Clin Pathol 69:122-7 (2016). Read more (PubMed: 26254281) »

See all 13 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (nasopharynx)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
No
Specification
nasopharynx
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 01 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cells)
Permeabilization
No
Specification
Hela cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Methanol
Username

Abcam user community

Verified customer

投稿 Sep 23 2015

Application
Western blot
Sample
Human Tissue lysate - whole (skin biopsie)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Loading amount
15 µg
Treatment
see below
Specification
skin biopsie
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 22 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Mesothelioma)
Specification
Mesothelioma
Treatment
siRNA and Radiation
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Saurabh Dayal

Verified customer

投稿 Sep 02 2014

This antibody has been tested for mouse in Western Blot although the image is not available.
At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform ...

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Thank you for contacting us.

We normally issue the discount code prior to buying and testing the product. However, in this case, I am happy to make an exception. I have just issued a new code.

Thank you for your Abreview on this p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount
30 µg
Specification
Mouse embryonic fibroblast
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 07 2012

Thank you very much for your interest in ab8416

We do not currently have an image of this antibody in western blot or IHC Therefore, I can offer a discount off a future purchase if you buy ab8416 now, use it in western blot or IHC and submit...

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Thank you for your enquiry. Ab8414 has been successfully tested for application in Western blotting and ab8416 has been successfully tested for application in Western blotting and IHC-P (see customer review). We currently do not have any information ab...

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Thank you for your question regarding the MW of DR5. The predicted MW of the human precursor protein is 47.8kDa according to SwissProt and the antibody ab8416 recognised a band just above the 55kDa MW marker in HeLa and K562 whole cell lysates. I h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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