製品の概要

  • 製品名Anti-DR5 antibody
    DR5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DR5
  • アプリケーション適用あり: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    KIEDHLLSSGKFMYLEGNAD

    , corresponding to internal sequence amino acids 388-407 of Human DR5 isoform 2. The same sequence is found in isoform 1 at amino acids 417-436.

  • ポジティブ・コントロール
    • HL60 or Jurkat whole cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab47179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. As reported in Younes et al. (2006).
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human DR5 peptide (ab8449).
Flow Cyt Use at an assay dependent concentration. As reported in KOYAMA (2003).



ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. As reported in Georgakis et al. (2005).

ターゲット情報

  • 機能Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
  • 組織特異性Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
  • 関連疾患Squamous cell carcinoma of the head and neck
  • 配列類似性Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fas like protein antibody
    • Apoptosis inducing protein TRICK2A/2B antibody
    • Apoptosis inducing receptor TRAIL R2 antibody
    • CD262 antibody
    • CD262 antigen antibody
    • Cytotoxic TRAIL receptor 2 antibody
    • Death domain containing receptor for TRAIL/Apo 2L antibody
    • Death receptor 5 antibody
    • DR5 antibody
    • KILLER antibody
    • KILLER/DR5 antibody
    • OTTHUMP00000123492 antibody
    • OTTHUMP00000123493 antibody
    • p53 regulated DNA damage inducible cell death receptor(killer) antibody
    • TNF related apoptosis inducing ligand receptor 2 antibody
    • TNF-related apoptosis-inducing ligand receptor 2 antibody
    • TNFRSF10B antibody
    • TR10B_HUMAN antibody
    • TRAIL R2 antibody
    • TRAIL receptor 2 antibody
    • TRAIL-R2 antibody
    • TRAILR2 antibody
    • TRICK2 antibody
    • TRICK2A antibody
    • TRICK2B antibody
    • TRICKB antibody
    • Tumor necrosis factor receptor like protein ZTNFR9 antibody
    • Tumor necrosis factor receptor superfamily member 10B antibody
    • Tumor necrosis factor receptor superfamily, member 10b antibody
    • ZTNFR9 antibody
    see all

Anti-DR5 antibody 画像

Anti-DR5 antibody (ab47179) 使用論文

This product has been referenced in:
  • Luan Z  et al. Rocaglamide overcomes tumor necrosis factor-related apoptosis-inducing ligand resistance in hepatocellular carcinoma cells by attenuating the inhibition of caspase-8 through cellular FLICE-like-inhibitory protein downregulation. Mol Med Rep 11:203-11 (2015). WB ; Human . Read more (PubMed: 25333816) »
  • Oh Y  et al. Regulation in the targeting of TRAIL receptor 1 to cell surface via GODZ for TRAIL sensitivity in tumor cells. Cell Death Differ 19:1196-207 (2012). WB ; Human . Read more (PubMed: 22240897) »

See all 8 Publications for this product

Product Wall

I am very pleased to hear you would like to accept our offer and test ab47179 in IHC-Fr. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the...

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Thank you very much for your interest in ab47179.

To our knowledge, ab47179 has not been tested in IHC on frozen tissue. There is no particular reason for this and it is likely that the antibody will work in this application, although not gu...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization Yes - TBST
Blocking step 2&NGS/2%BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Miss. Sandra Peternel

Verified customer

投稿 Jan 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"