Anti-DR4 抗体 [DR-4-02] - Low endotoxin, Azide free (ab14738)

Submit an Abreview Q&A (4)Specific References (2)

製品の概要

  • 製品名
    Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free
    DR4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DR-4-02] to DR4 - Low endotoxin, Azide free
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IP, Functional Studies, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Fusion protein (Human). Fusion protein containing the extracellular part of DR4 and the constant part of the heavy chain of the human IgG1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
Functional Studies
Flow Cyt
ICC/IF
  • 追加情報
    Flow Cyt: Use at a concentration of 3 - 5 µg/ml.
    FuncS: Use at an assay dependent dilution.Soluble antibody blocks DR4 receptor against TRAIL-induced apoptosis. Plastic-immobilized antibody induces apoptosis in sensitive cells.
    Recommended dilution of antibody: 2-3 µg/ml in cultivation medium
    Final concentration of TRAIL: 20-200 ng/ml
    Application note: It is recommended to add the antibody 15 min before addition of TRAIL.

    ICC/IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能
      Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
    • 組織特異性
      Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
    • 配列類似性
      Contains 1 death domain.
      Contains 3 TNFR-Cys repeats.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
      • 別名
        • Apo2 antibody
        • CD261 antibody
        • Cytotoxic TRAIL receptor antibody
        • Death receptor 4 antibody
        • DR4 antibody
        • NF related apoptosis-inducing ligand receptor 1 antibody
        • TNF receptor superfamily member 10a antibody
        • TNF-related apoptosis-inducing ligand receptor 1 antibody
        • TNFRSF10A antibody
        • TR10A_HUMAN antibody
        • TRAIL receptor 1 antibody
        • TRAIL-R1 antibody
        • TRAILR 1 antibody
        • TRAILR1 antibody
        • Tumor necrosis factor receptor superfamily member 10A antibody
        • Tumor necrosis factor receptor superfamily member 10a variant 2 antibody
        • Tumor necrosis factor receptor superfamily, member 10a antibody
        see all

      画像

      • Flow Cytometry - Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free (ab14738)
        Flow Cytometry - Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free (ab14738)
        Flow Cytometry analysis of DR4 expression on the surface of hematopoietic cell lines. Cells were stained with purified anti-DR4 antibodies (ab14738) followed by Goat anti-mouse IgG PE. Histograms: Red - Goat anti-mouse IgG PE (no primary antibody) Black - anti-DR4 comparative antibody Blue and Green - anti-DR4 (ab14738:DR-4-02) in duplicate staining
      • Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free (ab14738)
        Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody [DR-4-02] - Low endotoxin, Azide free (ab14738)
        Immunofluorescence staining (confocal microscopy) of HeLa human cervix carcinoma cell line transfected with DR4 expression plasmid using anti-human DR4 (ab14738:DR-4-02).

      参考文献

      This product has been referenced in:
      • Passante E  et al. Systems analysis of apoptosis protein expression allows the case-specific prediction of cell death responsiveness of melanoma cells. Cell Death Differ 20:1521-31 (2013). Read more (PubMed: 23933815) »
      • Franzen CA  et al. Matrix protein CCN1 is critical for prostate carcinoma cell proliferation and TRAIL-induced apoptosis. Mol Cancer Res 7:1045-55 (2009). Read more (PubMed: 19584265) »

      See all 2 Publications for this product

      ab14738 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      レビューと Q&A

      レビューを送る 質問を送る

      4 reviews or Q&A

      10
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      Kreuzreaktivität dieser Antikörper?

      Vielen Dank für Ihren Anruf.
      Ich habe nachgefragt ob und wie die Spezifität der folgenden Antikörper getestet wurde:
      ab108421:
      Dieser Antikörper wurde nicht experimentell auf die Kreuzreaktion mit anderen Trail Rezeptoren getestet. Ich k...

      Read More

      Hello, I have been using the above antibody to induce apoptosis in human cell lines. I wonder if you could tell me whether it cross-reacts with any of the other receptors, in particular DR5, or whether this is not known. Thank you!

      We can confirm that ab14738 is DR4 specific (at least as far as tested on transfectants). Please do not hesitate to contact us again if you need further information,

      I recently asked for advice on how to use your antibody (ab14738) to DR4 in order to induce apoptosis. In reply you sent me some information recommending using it to coat plastic dishes at a final concentration of 100 ug/ml. Since I only have 100 ug it...

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      The protocol I sent is correct- this is how the originator uses its antibody. However, I think the originators do not optimally titrate the antibody since they do not worry about the load (unlimited access of antibody), so you may find you will be able...

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      I'm looking into buying some of the above DR4 antibody to use to induce apoptosis in TRAIL-sensitive cells. According to your info sheet this requires plastic immobilisation - does this simply involve adding the solution to a plastic culture plate for ...

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      Thank you for your enquiry. Here is the protocol recommended for induction experiments: The antibody (at a final concentration 100 ug/ml) is spread onto the surface of plastic dish just to cover it (layer about 1-2 mm; e.g. 50 ul into a well of 96-...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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