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Fusion protein (Human) containing the extracellular part of DR4 and the constant part of the heavy chain of human IgG1.
Soluble antibody blocks DR4 receptor against TRAIL induced apoptosis. Plastic immobilized antibody induces apoptosis in sensitive cells.
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Our Abpromise guarantee covers the use of ab59047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use a concentration of 2 - 3 µg/ml. in cultivation medium. Final concentration of TRAIL: 20-200 ng/ml. Note: It is recommended that the antibody is added 15 min before the addition of TRAIL.|
|Flow Cyt||Use a concentration of 5 µg/ml. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|