製品の概要

  • 製品名
    Anti-DR4 antibody
    DR4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DR4
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    ASGTEAAAATPSKVWGSSAG

    , corresponding to amino acids 24/43 of Human DR4 (Peptide available as ab8447.)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • HeLa or Jurkatwhole cell lysate A 57 kDa band should be detected.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa.Can be blocked with Human DR4 peptide (ab8447).

ターゲット情報

  • 機能
    Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
  • 組織特異性
    Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
  • 配列類似性
    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Apo2 antibody
      • CD261 antibody
      • Cytotoxic TRAIL receptor antibody
      • Death receptor 4 antibody
      • DR4 antibody
      • NF related apoptosis-inducing ligand receptor 1 antibody
      • TNF receptor superfamily member 10a antibody
      • TNF-related apoptosis-inducing ligand receptor 1 antibody
      • TNFRSF10A antibody
      • TR10A_HUMAN antibody
      • TRAIL receptor 1 antibody
      • TRAIL-R1 antibody
      • TRAILR 1 antibody
      • TRAILR1 antibody
      • Tumor necrosis factor receptor superfamily member 10A antibody
      • Tumor necrosis factor receptor superfamily member 10a variant 2 antibody
      • Tumor necrosis factor receptor superfamily, member 10a antibody
      see all

    画像

    • Western blot - Anti-DR4 antibody (ab8415)
      Western blot - Anti-DR4 antibody (ab8415)
      All lanes : Anti-DR4 antibody (ab8415)

      Lane 1 : H: HeLa
      Lane 2 : K: K562
      Lane 3 : J: Jurkat whole cell lysate

      Observed band size: 57 kDa (why is the actual band size different from the predicted?)



      Western blot analysis of DR4 at 1:500 dilution.
    • Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody (ab8415)
      Immunocytochemistry/ Immunofluorescence - Anti-DR4 antibody (ab8415)

      Immunocytochemistry of DR4 in Hela cells with ab8415 at 2 μg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR4 antibody (ab8415)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR4 antibody (ab8415)This image is courtesy of an anonymous Abreview
      ab8415 staining DR4 in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 10 minutes at 16°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50 in 1.5% Normal horse serum) for 2 hours at 16°C. An undiluted Biotin-conjugated Human pan-specific polyclonal was used as the secondary antibody.

      See Abreview

    参考文献

    This product has been referenced in:
    • Köhler B  et al. Bid participates in genotoxic drug-induced apoptosis of HeLa cells and is essential for death receptor ligands' apoptotic and synergistic effects. PLoS ONE 3:e2844 (2008). WB ; Human . Read more (PubMed: 18665234) »

    See 1 Publication for this product

    ab8415 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    4 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DR4 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast)
    Specification
    Breast
    Fixative
    Formaldehyde
    Antigen retrieval step
    Other
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 1.5% · Temperature: 16°C
    Read More
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    Abcam user community

    Verified customer

    投稿 Mar 08 2013

    Western blot abreview for Anti-DR4 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast cell lines)
    Loading amount
    20 µg
    Specification
    Breast cell lines
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Nov 19 2010

    Customer Question: Does the DR4 antibody (Trail R-1) cross react with Trail-R2? Can you send me any data to support the specificity of your DR4 antibodies in Jurkat cell lysates?

    Thank you for your enquiry. It is highly unlikely that ab8415 cross-reacts with Trail-R2. To make sure, just perform a BLAST search with the immunizing sequence. Trail-R2 will not come up. Unfortunately, we do not have data supporting the specificity o...

    Read More

    If I utilize your antibodies for western blot, should I look for the N or the C terminal antibodies? I am interested in detecting the functional end of the protein.

    If you are looking for the functional end then it's best to look for the C-terminal as the N-terminal is usually cleaved.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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