製品の概要

  • 製品名Anti-DPYD antibody
    DPYD 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to DPYD
  • アプリケーション適用あり: WB, ICC/IF, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-111 of Human DPYD

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 111 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/100.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.
  • 組織特異性Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.
  • パスウェイAmino-acid biosynthesis; beta-alanine biosynthesis.
  • 関連疾患Defects in DPYD are the cause of dihydropyrimidine dehydrogenase deficiency (DPYD deficiency) [MIM:274270]; also known as hereditary thymine-uraciluria or familial pyrimidinemia. DPYD deficiency is a disease characterized by persistent urinary excretion of excessive amounts of uracil, thymine and 5-hydroxymethyluracil. Patients suffering from this disease show a severe reaction to the anticancer drug 5-fluorouracil. This reaction includes stomatitis, Leukopenia, thrombocytopenia, hair loss, diarrhea, fever, marked weight loss, cerebellar ataxia, and neurologic symptoms, progressing to semicoma.
  • 配列類似性Belongs to the dihydropyrimidine dehydrogenase family.
    Contains 3 4Fe-4S ferredoxin-type domains.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DHP antibody
    • DHPDHase antibody
    • Dihydropyrimidine dehydrogenase [NADP(+)] antibody
    • Dihydropyrimidine dehydrogenase [NADP+] antibody
    • Dihydropyrimidine dehydrogenase antibody
    • Dihydrothymine dehydrogenase antibody
    • Dihydrouracil dehydrogenase antibody
    • DPD antibody
    • DPYD antibody
    • DPYD_HUMAN antibody
    • MGC132008 antibody
    • MGC70799 antibody
    • OTTHUMP00000058954 antibody
    see all

Anti-DPYD antibody 画像



  • Predicted band size : 111 kDa
  • ab54797 at 10 ug/ml staining DPYD in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing HepG2 cells stained with ab54797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-DPYD antibody (ab54797) 使用論文

This product has been referenced in:
  • Offer SM  et al. microRNAs miR-27a and miR-27b directly regulate liver dihydropyrimidine dehydrogenase expression through two conserved binding sites. Mol Cancer Ther 13:742-51 (2014). WB . Read more (PubMed: 24401318) »
  • Offer SM  et al. Comparative functional analysis of DPYD variants of potential clinical relevance to dihydropyrimidine dehydrogenase activity. Cancer Res 74:2545-54 (2014). WB . Read more (PubMed: 24648345) »

See all 5 Publications for this product

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