製品の概要

  • 製品名Anti-Dppa4 antibody
    Dppa4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dppa4
  • 特異性The immunogen was designed to raise an antibody against mouse Dppa4. However, the antibody does not recognise a band of the expected size of Dppa4 in the mouse cell lysate tested. This may be because Dppa4 is not expressed in sufficient amounts for detection by Western Blotting in those cells. The immunogen also contained a sequence of 12 amino acids found in human Dppa4. The antibody recognises a band of approximately the expected size of Dppa4 in human embryonic stem cell lysate. Additionally, the immuogen contains a sequence of 12 consecutive amino acids which are found in human Dppa2 and a sequence of 13 amino acids of which 11 are found in mouse Dppa2. Therefore, it cannot be ruled out that the antibody recognizes Dppa2.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Synthetic peptide derived from within residues 200 to the C-terminus of Mouse Dppa4.

    (Peptide available as ab31647.)

  • ポジティブ・コントロール
    • Human Embryonic Stem Cell Lysate; Mouse Embryonic Stem Cell Lysate;

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.016% Sodium Azide
    Constituents: 0.8% BSA, 80mM Boric Acid, 60mM Sodium chloride, 4mM EDTA, 20mM Sodium borate, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab31648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).

ターゲット情報

  • 機能May be involved in the maintenance of active epigenetic status of target genes (By similarity). May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2410091M23Rik antibody
    • Developmental pluripotency associated 4 antibody
    • Developmental pluripotency associated protein 4 antibody
    • Developmental pluripotency-associated protein 4 antibody
    • Dppa 4 antibody
    • DPPA4 antibody
    • DPPA4_HUMAN antibody
    • FLJ10713 antibody
    see all

Anti-Dppa4 antibody 画像

  • All lanes : Anti-Dppa4 antibody (ab31648) at 1 µg/ml

    Lane 1 : Human Embryonic Stem Cell Lysate
    Lane 2 : Mouse Embryonic Stem Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa (possible IgG,glycosylated form,non-specific binding).

Anti-Dppa4 antibody (ab31648) 使用論文

This product has been referenced in:
  • Takahashi K  et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-72 (2007). WB ; Human . Read more (PubMed: 18035408) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"