Rabbit polyclonal to Dppa2
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Dppa2.
This antibody gave a positive signal in the following whole cell lysates: IOUD2; F9; Mouse EG; E14Tg2a.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
Use a concentration of 1 µg/ml.
Dppa2 is a SAP domain protein expressed only in pluripotent cells and the developing germ line.
cancer/testis antigen 100 antibody
Developmental Pluripotency Associated 2 antibody
Western blot - Dppa2 antibody (ab91318)
All lanes :
Anti-Dppa2 antibody (ab91318) at 1 µg/ml
Lane 1 :
IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 :
F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 :
Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 4 :
E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
37 kDa (
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Dppa2 antibody (ab91318)
ICC/IF image of ab91318 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91318, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab91318, DyLight® 488 goat anti-rabbit IgX (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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