製品の概要

  • 製品名Anti-Dppa2 antibody
    Dppa2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dppa2
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (NMEQMEPSVS STSDVKLEKP KKYNPGHLLQ TNEQFTAPQK ARCKIPALPL) of human Dppa2 (NP_620170).

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab83043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

ターゲット情報

  • 関連性Dppa2 is a SAP domain protein expressed only in pluripotent cells and the developing germ line.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • cancer/testis antigen 100 antibody
    • CT100 antibody
    • Developmental Pluripotency Associated 2 antibody
    • developmental pluripotency-associated gene 2 antibody
    • developmental pluripotency-associated protein 2 antibody
    • ECAT15-2 antibody
    • embryonic stem cell (ESC) associated transcript 15-2 antibody
    • PESCRG1 antibody
    • Pluripotent embryonic stem cell related protein 1 antibody
    • Pluripotent embryonic stem cell-related gene 1 antibody
    • Pluripotent embryonic stem cell-related gene 1 protein antibody
    see all

Anti-Dppa2 antibody 画像

  • Anti-Dppa2 antibody (ab83043) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

Anti-Dppa2 antibody (ab83043) 使用論文

ab83043 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample Marmoset (common) Tissue sections (Adult testis)
Specification Adult testis
Permeabilization No
Fixative Formaldehyde
Username

煜欽 林

Verified customer

投稿 Jul 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"