製品の概要

  • 製品名Anti-DPP6 antibody - Catalytic domain
    DPP6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DPP6 - Catalytic domain
  • 特異性This antibody will recognize the three longest forms of DPP6 but not the 353 amino acid form. It will not recognize the other family members.
  • アプリケーション適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Zebrafish
  • 免疫原

    Synthetic peptide based on the catalytic domain of human DPP6.

    (Peptide available as ab44770.)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab41811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr
WB
  • 追加情報WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 98 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性DPP6 (DPPX, also known as potassium channel accelerating factor Dppx) is a Type-II serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP6 has an Asp instead of Ser in the catalytic site. DPP6 is a member of a broader family of dipeptidyl peptidases including DPP4, FAP/Seprase, DPP2, DPP8, DPP9, DPP10, which have differing substrate specificity and tissue localizations. The surface-bound DPP6 is a homodimer, and cleavage of in the stalk region releases a shed form of DPP6. The shed is the form found in serum. DPP6 has been found in highest abundance in the brain, but also in the kidney, liver and lung.
    • 細胞内局在Cell Membrane; single-pass type II membrane protein
    • 参照データベース
    • 別名
      • Dipeptidyl aminopeptidase IV related protein antibody
      • Dipeptidyl aminopeptidase like protein 6 antibody
      • Dipeptidyl aminopeptidase related protein antibody
      • Dipeptidyl peptidase 6 antibody
      • Dipeptidyl peptidase IV like protein antibody
      • Dipeptidyl peptidase IV related protein antibody
      • Dipeptidylpeptidase 6 antibody
      • Dipeptidylpeptidase VI antibody
      • DPP VI antibody
      • DPPX antibody
      • VF2 antibody
      see all

    Anti-DPP6 antibody - Catalytic domain 画像

    • All lanes : Anti-DPP6 antibody - Catalytic domain (ab41811) at 1/1000 dilution

      Lane 1 : Mouse Brain
      Lane 2 : DU145 / IL1-beta Cell media


      Predicted band size : 98 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa (possible truncated form).
    • ab41811 staining DPP6 - Catalytic domain in zebrafish brain tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in paraformaldehyde, blocked with 2% serum for 2 hours at 24°C and then incubated with ab41811 at a 1/25 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.

      See Abreview

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    Anti-DPP6 antibody - Catalytic domain (ab41811) 使用論文

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Zebrafish Tissue lysate - whole (Neuronal tissue)
    Gel Running Conditions Reduced Denaturing (7.5%)
    Treatment RIPA buffer with protease inhibitor
    Specification Neuronal tissue
    Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 May 24 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Zebrafish Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
    Username

    Dr. Brent Bill

    Verified customer

    投稿 Sep 22 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"