製品の概要

  • 製品名
    Anti-DPM1 antibody [5C5A7]
    DPM1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5C5A7] to DPM1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Recombinant fragment

  • ポジティブ・コントロール
    • yeast lysate
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • 特記事項(精製)
    Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
  • ポリ/モノ
    モノクローナル
  • クローン名
    5C5A7
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab113686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 4 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

ターゲット情報

  • 機能
    Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.
  • パスウェイ
    Protein modification; protein glycosylation.
  • 関連疾患
    Defects in DPM1 are the cause of congenital disorder of glycosylation type 1E (CDG1E) [MIM:608799]. CDGs are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1E is an autosomal recessive disorder, characterized by severe developmental delay, hypotnia, seizures, and dysmorphic features.
  • 配列類似性
    Belongs to the glycosyltransferase 2 family.
  • 細胞内局在
    Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
    • 別名
      • CDGIE antibody
      • dolichol monophosphate mannose synthase antibody
      • Dolichol phosphate mannose synthase antibody
      • Dolichol-phosphate mannose synthase antibody
      • Dolichol-phosphate mannosyltransferase antibody
      • Dolichyl phosphate beta D mannosyltransferase antibody
      • dolichyl phosphate mannosyltransferase polypeptide 1 antibody
      • dolichyl phosphate mannosyltransferase polypeptide 1 catalytic subunit antibody
      • Dolichyl-phosphate beta-D-mannosyltransferase antibody
      • DPM synthase antibody
      • DPM1 antibody
      • DPM1_HUMAN antibody
      • Mannose P dolichol synthase antibody
      • Mannose-P-dolichol synthase antibody
      • MPD synthase antibody
      • MPDS antibody
      see all

    Anti-DPM1 antibody [5C5A7] 画像

    • Anti-DPM1 antibody [5C5A7] (ab113686) + Yeast whole cell lysate at 15 µg

      Predicted band size : 30 kDa

    プロトコール

    Anti-DPM1 antibody [5C5A7] (ab113686) 使用論文

    This product has been referenced in:
    • Simpkins JA  et al. Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration. Biol Open 5:689-97 (2016). WB . Read more (PubMed: 27142334) »
    • Cartwright BR  et al. Seipin performs dissectible functions in promoting lipid droplet biogenesis and regulating droplet morphology. Mol Biol Cell 26:726-39 (2015). Read more (PubMed: 25540432) »

    See all 3 Publications for this product

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    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (Saccharomyces cerevisiae)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Specification
    Saccharomyces cerevisiae
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Jun 09 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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