Anti-DPF2 抗体 [2283C1a] (ab74519)

製品の概要

  • 製品名
    Anti-DPF2 antibody [2283C1a]
    DPF2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2283C1a] to DPF2
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, Dot blotmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment from internal sequence of human DPF2.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab74519 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and then filtered through a 0.22µm membrane.
  • ポリ/モノ
    モノクローナル
  • クローン名
    2283C1a
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 44 kDa.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 機能
    May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the requiem/DPF family.
    Contains 1 C2H2-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • 細胞内局在
    Nucleus. Cytoplasm. 30% nuclear. 70% cytoplasmic.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis response zinc finger protein antibody
    • BAF45D antibody
    • BRG1-associated factor 45D antibody
    • D4 antibody
    • D4 zinc and double PHD fingers family 2 antibody
    • Double PHD fingers 2 antibody
    • double PHD fingers family 2 antibody
    • DPF 2 antibody
    • DPF2 antibody
    • MGC10180 antibody
    • Protein requiem antibody
    • REQ antibody
    • REQU_HUMAN antibody
    • Requiem antibody
    • Requiem apoptosis response zinc finger antibody
    • UBI D4 antibody
    • ubi-d4 antibody
    • UBID 4 antibody
    • UBID4 antibody
    • zinc and double PHD fingers family 2 antibody
    • Zinc finger protein ubi d4 antibody
    • Zinc finger protein ubi-d4 antibody
    see all

画像

  • Anti-DPF2 antibody [2283C1a] (ab74519) + Immunising recombinant protein

    Predicted band size: 44 kDa
    Observed band size: 39 kDa (why is the actual band size different from the predicted?)



    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

プロトコール

参考文献

ab74519 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
ChIP
Sample
Human Cell lysate - nuclear (Jurkat Cells)
Negative control
Input
Specification
Jurkat Cells
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
Abcam ab134942, which has worked for ChIP in the past
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投稿 Nov 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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