製品の概要

  • 製品名Anti-DP2 antibody
    DP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DP2
  • 特異性ab26260 recognises TFDP2.
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide: MIISTPQRLT SSGSVLIGSP YTPAPAMVTQ THIAEATGWV PGDRKRARKF, corresponding to a region within N terminal amino acids 1-50 of Human DP2

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.

ターゲット情報

  • 機能Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis.
  • 組織特異性High levels in heart and skeletal muscle. Also found in placenta, kidney, brain, lung and liver. The presence as well as the abundance of the different transcripts appear to vary significantly in different tissues and cell lines.
  • 配列類似性Belongs to the E2F/DP family.
  • 翻訳後修飾Ser-24 is probably phosphorylated by CDK2.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DP2 antibody
    • E2F dimerization partner 2 antibody
    • Tfdp2 antibody
    • TFDP2_HUMAN antibody
    • Transcription factor Dp 2 antibody
    • Transcription factor Dp-2 antibody
    see all

Anti-DP2 antibody 画像

  • Anti-DP2 antibody (ab26260) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50000 - 1/100000

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • Staining of human kidney tissue with ab26260 (4-8 ug/ml). Positive labelled epithelial cells of renal tubule are indicated with arrows. 400X magnification.

Anti-DP2 antibody (ab26260) 使用論文

ab26260 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (lymphocytes)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 10 µg
Specification lymphocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Dec 04 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"