Anti-Doublecortin 抗体 [EPR10935(B)] (ab167400)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10935(B)] to Doublecortin
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Doublecortin antibody [EPR10935(B)]
Doublecortin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10935(B)] to Doublecortin -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human Doublecortin aa 100-200. The exact sequence is proprietary. Synthetic peptide, corresponding to residues in Human Doublecortin (UniProt: O43602).
Database link: O43602 -
ポジティブ・コントロール
- Human fetal brain and C6 lysates; C6 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10935(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167400の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 49 kDa.
|
特記事項 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 49 kDa. |
ターゲット情報
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機能
Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration. -
組織特異性
Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. -
関連疾患
Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1). -
配列類似性
Contains 2 doublecortin domains. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1641 Human
- Entrez Gene: 13193 Mouse
- Entrez Gene: 84394 Rat
- Omim: 300121 Human
- SwissProt: O43602 Human
- SwissProt: O88809 Mouse
- SwissProt: Q9ESI7 Rat
- Unigene: 34780 Human
see all -
別名
- DBCN antibody
- Dbct antibody
- DC antibody
see all
画像
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All lanes : Anti-Doublecortin antibody [EPR10935(B)] (ab167400) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : C6 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 49 kDa -
Intracellular flow cytometric analysis of permeabilized C6 cells labeling Doublecortin with ab167400 at 1/100 dilution (red) or a Rabbit IgG (negative) (green).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab167400 は論文での使用が確認できていません。