製品の概要

  • 製品名
    Anti-DORFIN antibody
  • 製品の詳細
    Rabbit polyclonal to DORFIN
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (

    IFSTNTSSDNGLTSISKQIGDFIECPLCLLRHSKDRFPDIMTCHHRSCVD

    ) of Human DORFIN (NP_056250).

  • ポジティブ・コントロール
    • Human fetal brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre in peptide based assay is 1/1562500.

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.
  • 組織特異性
    Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the RBR family. RNF19 subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Membrane. Cytoplasm > cytoskeleton > centrosome. Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AA032313 antibody
    • DKFZP566B1346 antibody
    • Dorfin antibody
    • Double ring finger protein antibody
    • Double ring-finger protein antibody
    • E3 ubiquitin-protein ligase RNF19A antibody
    • p38 antibody
    • p38 protein antibody
    • Ring finger protein 19 antibody
    • RING finger protein 19A antibody
    • Ring IBR ring domain containing protein Dorfin antibody
    • RN19A_HUMAN antibody
    • RNF19 antibody
    • RNF19A antibody
    • Ubce7ip2 antibody
    • UIP117 antibody
    • XYbp antibody
    see all

画像

  • Anti-DORFIN antibody (ab83775) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 91 kDa
    Observed band size : 91 kDa

プロトコール

参考文献

ab83775 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
No
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Li-Or Zach

Verified customer

投稿 Dec 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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