The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
追加情報Is unsuitable for WB.
機能E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.
組織特異性Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system.
パスウェイProtein modification; protein ubiquitination.
配列類似性Belongs to the RBR family. RNF19 subfamily. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
細胞内局在Membrane. Cytoplasm > cytoskeleton > centrosome. Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
Ring IBR ring domain containing protein Dorfin antibody
Anti-DORFIN antibody 画像
Immunoprecipitation - DORFIN antibody (ab113276)
Detection of DORFIN by Western Blot of Immunprecipitate.
ab113276 at 1µg/ml staining DORFIN in HeLa whole cell lysate immunoprecipitated using ab113276 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
Anti-DORFIN antibody (ab113276) 使用論文
has not yet been referenced specifically in any publications.