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Our Abpromise guarantee covers the use of ab18548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 68 kDa.
An antibody raised against a similar peptide has been shown to react with a broad 80kD band in rat striatum and not cerebellum. The 80kD band comigrates with rat DAT labeled with an affinity ligand 125I-DEEP. The 80kD DAT band can be competed with the immunizing peptide. The same 80kD band (125I-DEEP labeled) can also be immunoprecipitated using the antiserum. Rat DAT contains various carbohydrate moieties and neuramnidase and N-Glycanase treatment reduces it size to ~55kD.
ab18548 has not yet been referenced specifically in any publications.