製品の概要
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製品名Anti-Dopamine Receptor D5 antibody
Dopamine Receptor D5 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Dopamine Receptor D5
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特異性Cross-reacts with D5 Dopamine Receptor(Ac23-35) 100%, D5 Dopamine Receptor 80%. Does not cross react with other dopamine receptors (D1, D2, D3, D4). This antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections, this includes cortex layer 5 and the parafascicular nucleus.
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アプリケーション適用あり: ICC/IF, Flow Cyt, WBmore details
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種交差性交差種: Rat
交差が予測される動物種: Human, Gorilla
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免疫原
Synthetic peptide: AcGNAVGGSAGAPP conjugated to KLH, corresponding to amino acids 23-35 of Dopamine Receptor D5
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ポジティブ･コントロール
- Whole rat brain homogenate rat brain sections
製品の特性
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製品の状態Lyophilised:Rehydrate the lyophilized antibody with 0.1 ml of PBS which contains 10 mg/ml BSA or with additional buffer (such as 10 mg/ml BSA in PBS) for more dilute antibody.
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保存方法Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: Whole serum -
Concentration information loading... -
精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab40656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/2500. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. | |
| WB | 1/200. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa). Blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
ターゲット情報
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機能Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
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組織特異性Neuron-specific, localized primarily within limbic regions of the brain.
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関連疾患Defects in DRD5 are a cause of benign essential blepharospasm (BEB) [MIM:606798]. BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness.
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配列類似性Belongs to the G-protein coupled receptor 1 family.
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細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 1816 Human
- Entrez Gene: 25195 Rat
- Omim: 126453 Human
- SwissProt: P21918 Human
- SwissProt: P25115 Rat
- Unigene: 380681 Human
- Unigene: 138110 Rat
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別名
- D(1B) dopamine receptor antibody
- D(5) dopamine receptor antibody
- D1 beta dopamine receptor antibody
see all
Anti-Dopamine Receptor D5 antibody 画像
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Immunocytochemistry/ Immunofluorescence - Anti-Dopamine Receptor D5 antibody (ab40656)ICC/IF image of ab40656 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40656, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
Anti-Dopamine Receptor D5 antibody (ab40656) 使用論文
ab40656 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"