製品の概要

  • 製品名
    Anti-Dopamine Receptor D4 antibody
    Dopamine Receptor D4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dopamine Receptor D4
  • 特異性
    Cross-reactivity: D4 Dopamine Receptor (176-185) 100% D4 Dopamine Receptor 60% D1 Dopamine Receptor (9-21) 0% D1 Dopamine Receptor 0% D2 Dopamine Receptor (272-282) 0% D2 Dopamine Receptor 0% D3 Dopamine Receptor (2-10) 0% D3 Dopamine Receptor 0% D5 Dopamine Receptor (23-35) 0% D5 Dopamine Receptor 0%
  • アプリケーション
    適用あり: ICC, WB, IHC-Pmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    D4 (Ac-176-185) covalently attached to a carrier protein.

  • ポジティブ・コントロール
    • Whole rat brain IF/ICC: PC12 cell line.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Lyophilized antisera.
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab20424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/10000.
WB 1/500. Detects a band of approximately 48-53 kDa (predicted molecular weight: 48 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 22155235

ターゲット情報

  • 機能
    Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻訳後修飾
    Polyubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex: polyubiquitination does not lead to degradation of DRD4 protein.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D(2C) dopamine receptor antibody
    • D(4) dopamine receptor (D(2C) dopamine receptor). antibody
    • D(4) dopamine receptor antibody
    • D4 dopamine receptor antibody
    • D4DR antibody
    • Dopamine D4 receptor antibody
    • DRD 4 antibody
    • Drd4 antibody
    • DRD4_HUMAN antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat gastric tissue labelling Dopamine Receptor D4 with ab20424 at 1/1000. Dopamine Receptor D4 was not found in the gastric mucosa (e - left) but was observed in the gastric enteric plexus (f - right). Paraffin sections were baked at 60°C for 2 hours, then deparaffinized in xylene for 20 minutes. Slides were rehydrated in descending concentrations of alcohol and transferred from 70% alcohol solution to distilled water for rinsing. Before staining, slides were pretreated with 10 mM citric acid (pH 6.0) at 92–98°C for 10 min and incubated in 10% normal serum and 0.1% Triton X-100 for 30 min. The sections were then incubated with the primary antibody, followed by the appropriate secondary antibody. Sections were washed in PBS and mounted in 90% glycerin PBS.

  • ab20424 stained PC12 cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20424 at 1/100) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

参考文献

This product has been referenced in:
  • Wang Q  et al. Cellular localization of dopamine receptors in the gastric mucosa of rats. Biochem Biophys Res Commun 417:197-203 (2012). IHC-P ; Rat . Read more (PubMed: 22155235) »
  • Krishnan B  et al. Dopamine receptor mechanisms mediate corticotropin-releasing factor-induced long-term potentiation in the rat amygdala following cocaine withdrawal. Eur J Neurosci 31:1027-42 (2010). Read more (PubMed: 20377617) »

See all 2 Publications for this product

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